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Differential effects of NGF, FGF, EGF, cAMP, and dexamethasone on neurite outgrowth and sodium channel expression in PC12 cells

Pollock, Jonathan D. and Krempin, Marie and Rudy, Bernardo (1990) Differential effects of NGF, FGF, EGF, cAMP, and dexamethasone on neurite outgrowth and sodium channel expression in PC12 cells. Journal of Neuroscience, 10 (8). pp. 2626-2637. ISSN 0270-6474. PMCID PMC6570261. https://resolver.caltech.edu/CaltechAUTHORS:20191104-092443273

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Abstract

PC12 cells are a pheochromocytoma cell line that can be made to differentiate into sympatheticlike neurons by nerve growth factor (NGF). An essential component of the NGF-induced differentiation is the development of action potentials and sodium channels. Using whole-cell clamp we have confirmed that NGF produces a 5- to 6-fold increase in sodium channel density. The sodium channels induced by NGF are not different from those in cells not treated with NGF and are similar to those in other cell types. Basic fibroblast growth factor (FGF), another growth factor that causes PC12 cells to differentiate into sympathetic-like neurons, also produces a 5- to 6-fold increase in sodium current density with channels indistinguishable from those in PC12 cells treated and not treated with NGF. Basic FGF produces the same or somewhat larger increase in sodium channel density but much less neurite outgrowth. In contrast, epidermal growth factor does not produce neurite outgrowth but induces a small, reproducible increase in sodium channel density. Cyclic AMP produces spike-like processes but not neurites and results in a decrease in sodium current and sodium current density. Dexamethasone, a synthetic glucocorticoid, inhibits the increase in sodium current and sodium current density but does not antagonize the neurite outgrowth induced by NGF. Thus, although the increase in sodium channel expression induced by NGF and basic FGF parallels the changes in morphology that lead to neurite outgrowth, it clearly does not depend on them. The results show that different aspects of neuronal differentiation might be independently regulated by the microenvironment.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1523/jneurosci.10-08-02626.1990DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6570261PubMed CentralArticle
Additional Information:© 1990 Society for Neuroscience. Received Aug. 14, 1989; revised Mar. 13, 1990; accepted Mar. 23, 1990. Part of this work was done in the laboratory of Dr. Mark Tanouye. We wish to thank Mark Tanouye for his most generous support of this work. We would also like to thank Dr. Norman Davidson for allowing us to use his tissue culture facilities. This work was supported by NRSA Fellowship 2 F 32 NS07903-03 to J.D.P., by NIH Grant GM 26976 to B.R. and by the Pfeiffer Research Foundation and USPHS Grant NS21327 to Dr. Mark Tanouye.
Funders:
Funding AgencyGrant Number
NIH Postdoctoral Fellowship2 F 32 NS07903-03
NIHGM 26976
Pfeiffer Research FoundationUNSPECIFIED
NIHNS21327
Issue or Number:8
PubMed Central ID:PMC6570261
Record Number:CaltechAUTHORS:20191104-092443273
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191104-092443273
Official Citation:Differential effects of NGF, FGF, EGF, cAMP, and dexamethasone on neurite outgrowth and sodium channel expression in PC12 cells. JD Pollock, M Krempin, B Rudy. Journal of Neuroscience 1 August 1990, 10 (8) 2626-2637; DOI: 10.1523/JNEUROSCI.10-08-02626.1990
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:99643
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Nov 2019 18:35
Last Modified:04 Nov 2019 18:35

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