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G Proteins Modulate D2 Receptor-Coupled K(ATP) Channels in Rat Dopaminergic Terminals

Neusch, Clemens and Runde, Doris and Moser, Andreas (2000) G Proteins Modulate D2 Receptor-Coupled K(ATP) Channels in Rat Dopaminergic Terminals. Neurochemical Research, 25 (12). pp. 1521-1526. ISSN 0364-3190. doi:10.1023/a:1026620316090.

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The presynaptic dopamine (DA) D2 receptor-mediated regulation of ATP-sensitive potassium (K⁺_(ATP)) channels was examined in slices of the rat caudate-putamen. When slices were incubated with the specific D2 receptor antagonist (−)-sulpiride (SLP), a concentration-dependent increase of extracellular DA release was observed. SLP-induced enhancement was completely antagonized by coincubation with the K⁺_(ATP) channel opener diazoxide (DIA). Treatment of slices with the D2 receptor agonist quinpirole (QUI) almost completely inhibited DA outflow induced by the K⁺_(ATP) channel blocker butanedione-monoxime (BDM). Coincubation of SLP and guanosine triphosphate (GTP) or its non-hydrolizable analogue guanylyl-5′-imidodiphosphate [Gpp(NH)p], significantly reduced the SLP-induced effect on DA levels. Furthermore, we observed that BDM-induced DA outflow was markedly inhibited by G protein activators suggesting an additional receptor-independent regulation of K⁺_(ATP) channel gating. Our results suggest that PTX-sensitive G proteins are involved in the signal transduction between D2 receptors and K⁺_(ATP) channels. Furthermore, K⁺_(ATP) channels can be modulated in a receptor-independent mechanism by G protein activators.

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Additional Information:© 2000 Plenum Publishing Corporation. Accepted August 2, 2000.
Subject Keywords:D2 receptor; G protein; guanosine nucleotides; ATP-sensitive potassium channel; caudateputamen
Issue or Number:12
Record Number:CaltechAUTHORS:20191112-100802592
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Official Citation:Neusch, C., Runde, D. & Moser, A. Neurochem Res (2000) 25: 1521.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:99803
Deposited By: Tony Diaz
Deposited On:12 Nov 2019 23:01
Last Modified:16 Nov 2021 17:49

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