Chimeric chemosensory transducers of Escherichia coli
The tar and tsr genes of Escherichia coli encode homologous transducer proteins that mediate distinct chemotactic responses. We report here the construction of two tasr chimeric genes in which the 5' coding region of the tar gene is fused to the 3' coding region of the tsr gene at either of two conserved restriction sites. Both chimeric genes code for chemotactically functional proteins. Results of analyses of behavior and methylation in cells carrying the chimeric genes support existing models for the disposition of transducer domains across the cell membrane and reveal that the receptors for internal pH map in a specific region of the COOH-terminal (cytoplasmic) domain.
Additional Information© 1985 by the National Academy of Sciences. Contributed by Howard C. Berg, October 22, 1984. We thank N. Mutoh, J. E. Segall, A. Ishihara, R. M. Macnab, K. Oosawa, M. F. Bruist, and E. M. Phizicky for their help. This work was supported by National Institutes of Health Grants AI16478 (to H.C.B.) and AI14988 (to M.I.S.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Published - KRIpnas85.pdf