Published November 2009
| Version Accepted Version + Supplemental Material
Journal Article
Open
Crystal structure of TNFα complexed with a poxvirus MHC-related TNF binding protein
-
1.
California Institute of Technology
Abstract
The poxvirus 2L protein binds tumor necrosis factor-α (TNFα) to inhibit host antiviral and immune responses. The 2.8-Å 2L–TNFα structure reveals three symmetrically arranged 2L molecules per TNFα trimer. 2L resembles class I major histocompatibility complex (MHC) molecules but lacks a peptide-binding groove and β2-microglobulin light chain. Overlap between the 2L and host TNF receptor-binding sites on TNFα rationalizes 2L inhibition of TNFα–TNF receptor interactions and prevention of TNFα-induced immune responses.
Additional Information
© 2009 Nature Publishing Group, a division of Macmillan Publishers Limited. Received 29 April; accepted 26 August; published online 18 October 2009. Diffraction data were collected at the Stanford Synchrotron Radiation Laboratory. We thank the Caltech Protein Expression Center and the Gordon and Betty Moore Foundation for support of the Molecular Observatory at Caltech. This work was supported by a Life Sciences Research Foundation Fellowship (Z.Y.) and the Howard Hughes Medical Institute. Accession codes. Protein Data Bank: Coordinates and X-ray crystallographic data for the 2L–TNFalpha complex have been deposited with accession code 3IT8. Author Contributions: Z.Y. and A.P.W. performed the experiments; Z.Y., A.P.W. and P.J.B. analyzed and interpreted that data; P.J.B. oversaw the project.Attached Files
Accepted Version - nihms172308.pdf
Supplemental Material - nsmb.1683-S1.pdf
Files
nihms172308.pdf
Files
(6.1 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:0804bbc48a9e7475d05fa0939d17b374
|
1.8 MB | Preview Download |
|
md5:a9a2b258deac4a31234cfb1c7d5cce2c
|
4.3 MB | Preview Download |
Additional details
Identifiers
- PMCID
- PMC2819277
- Eprint ID
- 16781
- DOI
- 10.1038/nsmb.1683
- Resolver ID
- CaltechAUTHORS:20091123-100928279
Related works
- Describes
- 10.1038/nsmb.1683 (DOI)
Funding
- Caltech Protein Expression Center
- Gordon and Betty Moore Foundation
- Life Sciences Research Foundation
- Howard Hughes Medical Institute (HHMI)
Dates
- Created
-
2009-11-24Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field