LOCUS MDS 88028-90092 rK1m between cra&mraZ 2869 bp ds-DNA linear SYN 13-SEP-2016
DEFINITION synthetic linear DNA
ACCESSION .
VERSION .
KEYWORDS MDS 88028-90092 rK1m between cra&mraZ
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2869)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, Sep 13, 2016 from SnapGene 3.2.1
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2869
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..1005
/label=cra
/note="cra"
misc_feature 1008..1037
/label=protoSpacer3
/note="protoSpacer3"
misc_feature 1038..1040
/label=PAM3
/note="PAM3"
misc_feature 1039..1176
/label=promoter
/note="promoter"
misc_feature 1177..1551
/label=rpsL
/note="rpsL"
misc_feature 1563..2357
/label=KanR
/note="KanR"
misc_feature complement(2358..2360)
/label=PAM4
/note="PAM4"
misc_feature complement(2361..2390)
/label=protoSpacer4
/note="protoSpacer4"
misc_feature 2373..2410
/label=5' mRNA
/note="5' mRNA"
gene 2411..2869
/gene="mraZ"
/gene_synonym="b0081; ECK0082; JW0079"
/locus_tag="ECMDS42_0074"
/label=mraZ
/note="mraZ"
/db_xref="GeneID:14967515"
ORIGIN
1 gtgaaactgg atgaaatcgc tcggctggcg ggagtgtcgc ggaccactgc aagctatgtt
61 attaacggca aagcgaagca ataccgtgtg agcgacaaaa ccgttgaaaa agtcatggct
121 gtggtgcgtg agcacaatta ccacccgaac gccgtggcag ctgggcttcg tgctggacgc
181 acacgttcta ttggtcttgt gatccccgat ctggagaaca ccagctatac ccgcatcgct
241 aactatcttg aacgccaggc gcggcaacgg ggttatcaac tgctgattgc ctgctcagaa
301 gatcagccag acaacgaaat gcggtgcatt gagcaccttt tacagcgtca ggttgatgcc
361 attattgttt cgacgtcgtt gcctcctgag catccttttt atcaacgctg ggctaacgac
421 ccgttcccga ttgtcgcgct ggaccgcgcc ctcgatcgtg aacacttcac cagcgtggtt
481 ggtgccgatc aggatgatgc cgaaatgctg gcggaagagt tacgtaagtt tcccgccgag
541 acggtgcttt atcttggtgc gctaccggag ctttctgtca gcttcctgcg tgaacaaggt
601 ttccgtactg cctggaaaga tgatccgcgc gaagtgcatt tcctgtatgc caacagctat
661 gagcgggagg cggctgccca gttattcgaa aaatggctgg aaacgcatcc gatgccgcag
721 gcgctgttca caacgtcgtt tgcgttgttg caaggagtga tggatgtcac gctgcgtcgc
781 gacggcaaac tgccttctga cctggcaatt gccacctttg gcgataacga actgctcgac
841 ttcttacagt gtccggtgct ggcagtggct caacgtcacc gcgatgtcgc agagcgtgtg
901 ctggagattg tcctggcaag cctggacgaa ccgcgtaagc caaaacctgg tttaacgcgc
961 attaaacgta atctctatcg ccgcggcgtg ctcagccgta gctaagccgc gaacaaaaat
1021 acgcgccagg tgaaaattgg cctggtgatg atggcgggat cgttgtatat ttcttgacac
1081 cttttcggca tcgccctaaa attcggcgtc ctcatattgt gtgaggacgt tttattacgt
1141 gtttacgaag caaaagctaa aaccaggagc tatttaatgg caacagttaa ccagctggta
1201 cgcaaaccac gtgctcgcaa agttgcgaaa agcaacgtgc ctgcgctgga agcatgcccg
1261 caaaaacgtg gcgtatgtac tcgtgtatat actaccactc ctaaaaaacc gaactccgcg
1321 ctgcgtaaag tatgccgtgt tcgtctgact aacggtttcg aagtgacttc ctacatcggt
1381 ggtgaaggtc acaacctgca ggagcactcc gtgatcctga tccgtggcgg tcgtgttaaa
1441 gacctcccgg gtgttcgtta ccacaccgta cgtggtgcgc ttgactgctc cggcgttaaa
1501 gaccgtaagc aggctcgttc caagtatggc gtgaagcgtc ctaaggctta aggaggacaa
1561 tcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
1621 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
1681 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
1741 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
1801 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
1861 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
1921 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
1981 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
2041 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
2101 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
2161 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
2221 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
2281 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
2341 ttgacgagtt cttctgaccg cggtcagtgg gaaattgtgg ggcaaagtgg gaataagggg
2401 tgaggctggc atgttccggg gagcaacgtt agtcaatctc gacagcaaag ggcgcttatc
2461 agtgcctacc cgttatcggg aacagctgct tgagaacgct gccggtcaaa tggtttgcac
2521 cattgacatt tatcacccgt gcctgctgct ttaccccctg cctgaatggg aaattatcga
2581 gcaaaaatta tcgcgtctgt cgagcatgaa cccggttgag cgccgtgtgc agcgcctact
2641 gttaggtcat gccagcgaat gtcagatgga tggcgcaggt cgattgttaa tcgcgccagt
2701 actgcggcaa catgccgggc tgacaaaaga agtgatgctg gttggacagt tcaacaagtt
2761 tgagctgtgg gatgaaacaa cctggcatca acaggtcaag gaagatatcg acgcagagca
2821 gttggctacc ggagacttat cggagcgact gcaggacttg tctctataa
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