LOCUS pKW21_MB1amp_Spacers12 2519 bp ds-DNA circular SYN 05-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pKW21_MB1amp_Spacers12
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2519)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, Sep 13, 2016 from SnapGene 3.2.1
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2519
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 104..208
/label=AmpR promoter
/note="AmpR promoter"
misc_feature 209..1069
/label=AmpR ORF
/note="AmpR ORF"
misc_feature 1240..1828
/label=pMB1 Ori
/note="pMB1 Ori"
misc_feature 2032..2075
/label=rrnB T1 terminator
/note="rrnB T1 terminator"
misc_feature 2102..2439
/note="CRISPR insert"
misc_feature 2114..2138
/label=promoter predicted
/note="promoter predicted"
misc_feature 2240..2275
/label=direct repeat 1
/note="direct repeat 1"
misc_feature 2276..2305
/label=protoSpacer1
/note="Spacer1"
misc_feature 2306..2341
/label=direct repeat 1(1)
/note="direct repeat 2"
misc_feature 2342..2371
/label=protoSpacer2
/note="Spacer2"
misc_feature 2372..2407
/label=direct repeat 1(2)
/note="direct repeat 3"
ORIGIN
1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgcctaggtc
1981 tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
2041 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctctagca cgcgtaccat
2101 gggatcctat ttcttaataa ctaaaaatat ggtataatac tcttaataaa tgcagtaata
2161 caggggcttt tcaagactga agtctagctg agacaaatag tgcgattacg aaatttttta
2221 gacaaaaata gtctacgagg ttttagagct atgctgtttt gaatggtccc aaaaccgcgg
2281 cttagctacg gctgagcacg cccctgtttt agagctatgc tgttttgaat ggtcccaaaa
2341 cgtgggaata aggggtgagg ctggcatgcc tgttttagag ctatgctgtt ttgaatggtc
2401 ccaaaacttc agcacactga gacttgttga gttgaattcg gtcagtgcgt cctgctgatg
2461 tgctcagtat ctctatcact gatagggatg tcaatctcta tcactgatag ggactcgag
//