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Published 1994 | metadata_only
Journal Article

The production of nitric oxide in endothelial cells by amphiphiles


Lysophosphatidylcholine, an endogenous detergent is an endothelium-dependent smooth muscle relaxant, which acts through the release of nitric oxide. It is known to activate a number of membrane-bound enzymes. Because of the relationship between detergent action, relaxation of endothelium-intact rabbit aortic strips and the release of nitric oxide, we considered the possibility that other amphiphiles also produce nitric oxide from endothelial cells. We therefore investigated the effect of digitonin on relaxation of precontracted rabbit aortic strips and the release of nitric oxide from freshly harvested bovine endothelial cells as determined by chemiluminescence. We found that both digitonin and LPC release nitric oxide and that this process is inhibited by the NO synthase inhibitor Nω-Nitro-L-Arginine Methyl Ester (L-NNAME).

Additional Information

© 1994 Elsevier Science Ltd. Received in final form January 25, 1994. The original work for this study was supported by grants from the Council for Tobacco Research, USA, Inc., New York and The Margaret W. and Herbert Hoover, Jr. Foundation, Pasadena, California. Dr. Conforto is the recipient of the Boswell Fellowship, jointly administered by the Huntington Medical Research Institutes and the California Institute of Technology. The authors would like to thank Matthew Vanasco and Alice Alegria for technical assistance and Linda Dechaine for secretarial help.

Additional details

August 22, 2023
August 22, 2023