The human blue opsin promoter directs transgene expression in short-wave cones and bipolar cells in the mouse retina
Transgenic mouse lines were generated using either 3.8 or 1.1 kb of 5' upstream flanking sequence from the human blue opsin gene fused to the lacZ or human growth hormone reporter gene. Mice were analyzed for appropriate cell-specific and developmental expression patterns. In 13 independently derived lines of animals, transgene expression was limited to photoreceptor and inner nuclear layer cells. Photoreceptors were identified as cone cells based on morphological criteria and colocalization of transgene expression with the cone-associated marker, peanut agglutinin lectin. More specifically, transgene-positive photoreceptors were identified as short-wave cone cells (S-cones) by using the short-wave color opsin-specific antibody, OS-2. Reporter-gene-positive cells of the inner nuclear layer were identified as bipolar cells based on morphological criteria. Transgenes and the endogenous mouse short-wave opsin gene were transcriptionally coactivated at embryonic day 13. These results show that 3.8 or 1.1 kb of human blue opsin upstream flanking sequences are capable of directing expression in short-wave cone cells in a spatially and temporally appropriate fashion and that the human blue opsin gene is the homologue of the short-wave-sensitive pigment, S-opsin, in the short-wave cones of the mouse retina. Expression in the bipolar cells may reflect regulatory mechanisms that are common to these cells and to the cone photoreceptors.
© 1994 by the National Academy of Sciences. Contributed by M.I. Simon, November 3, 1993. We thank Dr. J.P. Revel for assistance with confocal microscopy. This work was supported by public funds from the National Institutes of Health (NEI/EY04950) (E.B.), National Institutes of Health Program Project Grant AG 97687 (M.I.S.), National Institutes of Health fellowship (NEI/EY0640502) (J.C.), and grants from the George Gund/National Retinitis Pigmentosa Foundation (J.L. and M.I.S., E.B. and M.I.S.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Published - SLEpnas94.pdf