Supplementary Figure 2.
Photophysical characterization of BFP variants
Quantum
y
ield
vs. quinine sulfate standard
0
1000000
2000000
3000000
4000000
5000000
6000000
7000000
0
0.02
0.04
0.06
0.08
Absorbance
Integrated Fluorescence
Quinine Sulfate
0
500000
1000000
1500000
2000000
0
0.01
0.02
0.03
0.04
Absorbance
Integrated Fluorescence
BFP
BFP(F64L/V163A)
A5
Azurite
Extinction
c
oefficient
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0
5
10
15
Concentration (M x 10
-6
)
Absorbance (cm
-1
)
BFP
BFP(F64L/V163A)
A5
Azurite
Sample
QY Slope
QY
EC
Quinine Sulfate
113,978,776
0.53
1
n/a
BFP
73,957,467
0.34
27,100
BFP(F64L/V163A)
58,370,428
0.27
43,500
A5
104,180,124
0.48
27,400
Azurite
118,817,932
0.55
26,200
1.
Lakowicz, J.R. Principles of Fluorescence Spectroscopy, Edn. 2. (Kluwer
Academic/Plenum Publishers, New York; 1999).