Supplementary Figure
6.
Non
-denaturing polyacrylamide gel
electrophoresis of BFP
variants. (Top) Coomassie staining and
(Bottom) UV illumination prior to staining.
(1) 5
μ
L of
0.5 mg/mL (
20
μ
M)
, (2) 5
μ
L of
5 mg/mL (
200
μ
M)
protein
. (a) BFP F64L/V163A,
(b) BFP.A5, and (c) Azurite.
1a 1b 1c 2a 2b 2c
1
Supplementary Figure 6.
BFP crystal structure showing the BFP.1B3 mutations near the
chromophore. Image made using MOE.
Arg224
Ile150
Val224
Val150
Arg224
Ile150
Val224
Val150