Collisional Activation of Large Molecules Is an Efficient Process
It is a common perception that as molecules become larger they become inherently more difficult to activate and dissociate in the gas phase, especially under low energy collision induced dissociation (CID) conditions. This difficulty has been attributed both to the inefficiency of the collisional activation process and to the requirement of higher levels of internal excitation to induce dissociation. A serious consequence of this supposition is the implied difficulty of using CID methods to sequence peptides and proteins.
© 1994 American Chemical Society. Received March 24, 1994. We gratefully acknowledge the financial support of E.M.M. from a Rainin Fellowship and a NIH-NRSA Human Genome Fellowship, of S.C. from a NIH-NRSA traineeship in biotechnology, and of M.T.R. from a California Institute of Technology Consortium grant. We are indebted to the Beckman Foundation and Institute for support of the research facilities. This work was supported in part by the NSF under Grant CHE-9108318. Funds for instrument development have also been provided by ARPA and the DOD-URI program (ONR-N0014-92-J-1901).