Inorg. Chem., 1998, 37(20), 5198-5210, DOI:10.1021/ic9801948
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Copyright © 1998 American Chemical Society
©
1998
American
Chemical
Society,
Inorg.
Chem.,
Franklin
ic9801948
Supporting
Info
Page
1
FIGURE
CAPTIONS
Figure
S1.
Circular
dichroism
of
10
pM
A-Rh
(solid)
and
10
gM
A-Rh
(dashed)
in
(a)
the
absence
of
[poly(dA-dT)1
2
and
in
(b)
the
presence
of
[poly(dA-dT)]
2
(0.25
mM
bp).
Spectra
were
recorded
in
a
5
mM
sodium
phosphate,
50
mM
NaC1,
pH
7.0
buffer.
(c)
Figure
reproduced
from
reference
13
with
permission.
Figure
S2.
Expanded
TMe
and
H2',2"
vs.
aromatic
region
of
the
2D-NOESY
Contours
in
90:10
H
2
0:D
2
0
for
(counterclockwise
from
top
left)
D1/D2
alone,
Rh'+D1/D2,
Ru+D1/D2,
and
Rh'+Ru+D1/D2.
Solutions
were
0.5
mM
in
DNA
duplex,
0.45
mM
in
Rh'
and
0.45
mM
in
Ru
in
10
mM
sodium
phosphate,
pH
7.0.
Figure
S3.
2D-NOESY
Contour
in
90:10
H
2
0:D
2
0
for
D1/D2
alone.
Solutions
were
0.5
mM
in
DNA
duplex
in
10
mM
sodium
phosphate,
pH
7.0.
Figure
S4.
2D-NOESY
Contour
in
90:10
H
2
0:D
2
0
for
Rh'+DI/D2.
Solutions
were
0.5
mM
in
DNA
duplex
and
0.45
mM
in
Rh',
10
mM
sodium
phosphate,
pH
7.0.
Figure
SS.
2D-NOESY
Contour
in
90:10
H
2
0:D
2
0
for
Ru+D1/D2.
Solutions
were
0.5
mM
in
DNA
duplex,
0.45
mM
in
Ru,
10
mM
sodium
phosphate,
pH
7.0.
Figure
S6.
2D-NOESY
Contour
in
90:10
H
2
0:D
2
0
for
Rh'+Ru+D1/D2.
Solutions
were
0.5
mM
in
DNA
duplex,
0.45
mM
in
Rh'
and
0.45
mM
in
Ru
in
10
mM
sodium
phosphate,
pH
7.0.
34
Figure
Sla
450
Wavelength
(nm)
1 5
1 0
5
0
-5
-10
-15
400
500
S.0
IC
Figure
S1b
20
1
5
1
50
0
-5IN
-10
/
-15
400
450
500
Wavelength
(nm)
©
1998
American
Chemical
Society,
Inorg.
Chem.,
Franklin
ic9801948
Supporting
Info
Page
4
Figure
S1c
(a)
12P1(Ru+Rh)
0
30
b)
50PI(Ru+Rh)
**4(bc)
(c)
50PIRu+50P/Rh
(b)0
5OPIRu
*
25PIRu
20
5OP/Rh
-
25P/Rh
10
-
1-
's0
400
450
500
600
5OP/Ru
50P
h
-10
350
400
450
500
550
60
Wavelength
I
nm
Figure
1.
Circular
dichroism
spectra
showing
short-distance
interac-
tions
between
A-[Ru(phen)
2
dppz]
2
+
(RU)
and
A-[Rh(phi)
2
bipy]"
(RH)
at
low
DNA-binding
densities.
(a)
12P/(Ru
+
Rh)
(solid
line):
Spectrum
of
A-RU
(10
1
M)
and
A-RH
(10
/M)
in
the
presence
of
[poly(dA-
dT)]
2
(AT,
120
uM
nucleotides).
(b)
50P/(Ru
+
Rh)
(solid
line):
Spectrum
of
A-RU
(10
jM)
and
A-RH
(10
uM)
in
the
presence
of
AT
(500
MM
nucleotides).
50P/Ru
(dotted
line):
Spectrum
of
A-RU
(10
MM)
in
the
presence
of
AT
(500
uM
nucleotides).
50P/Rh
(dashed
line):
Spectrum
of
A-RH
(10
MM)
in
the
presence
of
AT
(500
/M
nucleotides).
(c)
50P/Ru
+
50P/Rh
(solid
line):
Sum
of
the
spectra
50P/Ru
and
50P/Rh.
Inset
shows
the
difference
spectra
a-c
(dotted
line)
and
b-c
(solid
line)
and
the
difference
spectrum
of
A-RU
(10
IM)
in
the
presence
of
different
concentrations
of
AT
(500
and
250
puM,
dotted
line)
and
the
difference
spectrum
of
A-RH
(10
jM)
under
the
same
conditions
(solid
line).
The
circular
dichroism
has
been
normalized
to
molar
differential
absorptivity
(AE).
Reprinted
from
Journal
of
the
American
Chemical
Society,
Vol.
119,
No.
6,
1454-1455,
1997
(enlarged).
©
1998
American
Chemical
Society,
Inorg.
Chem.,
Franklin
ic9801948
Supporting
Info
Page
5
Figure
S2.
Rh'+Ru+DNA
DNA
00
0
o
0
0o
20
00
Ln
0
0
N
0
8
7o.5
7.0
8.5
8.o
75
7.0
Rh'+DNA
Ru+DNA
0
0
00
0
0
0
A
0t
@~
Q
8.5
.0
75
7.
0
7.0
i.5
8.0
7.5
©
1998
American
Chemical
Society,
Inorg.
Chem.,
Franklin
ic9801948
Supporting
Info
Page
6
Figure
S3.
wdd
09
06
Cecet
U
*%:
-~:~*'~
-
-**
&
-
*~
~
-
-
-
0
0.
Cc,
a
I
-
-4&
QQ
-AQ
--
.-
10
-6-
'-
.6
-
-
'"
a-
-I.
-
9
IC
..
a.
D
1998
American
Chemical
Society,
Inorg.
Chem.,
Franklin
ic9801948
Supporting
Info
Page
7
Figure
S4.
wdd
Cl
........
.
.......................
..........
.
.
.
.
.
.
.
.
.
..
..
.
.
.
.
.
.
.
.
........
Qx
C~
D
1998
American
Chemical
Society,
Inorg.
Chem.,
Franklin
ic9801948
Supporting
Info
Page
8
Figure
S5.
wjdd'
000'9
0'6
w
0o
1998
American
Chemical
Society,
Inorg.
Chem.,
Franklin
ic9801948
Supporting
Info
Page
9
Figure
S6.
udd
o'C
0*9
o06
0
i2
.
.
*:
o
n
e