Blood pulse wave velocity measured by photoacoustic microscopy
Blood pulse wave velocity (PWV) is an important indicator for vascular stiffness. In this letter, we present electrocardiogram-synchronized photoacoustic microscopy for in vivo noninvasive quantification of the PWV in the peripheral vessels of mice. Interestingly, strong correlation between blood flow speed and ECG were clearly observed in arteries but not in veins. PWV is measured by the pulse travel time and the distance between two spot of a chose vessel, where simultaneously recorded electrocardiograms served as references. Statistical analysis shows a linear correlation between the PWV and the vessel diameter, which agrees with known physiology. Keywords: photoacoustic microscopy, photoacoustic spectroscopy, bilirubin, scattering medium.
Additional Information© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE). The authors appreciate the close reading of the manuscript by Profs. James Ballard, Seema Dahlheimer, and Lynnea Brumbaugh. Thanks to Amy Winkler and Brian Soetikno for helpful discussions. Thanks to Di Lang for experimental assistance. This work was sponsored by National Institutes of Health Grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959. L.V.W. has financial interests in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. K. Maslov has a financial interest in Microphotoacoustics, Inc.
Published - 85812C.pdf