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Published February 23, 2010 | Published
Book Section - Chapter Open

Optical-resolution photoacoustic microscopy of amyloid-β deposits in vivo


Advances in high-resolution imaging have permitted microscopic observations within the brains of living animals. Applied to Alzheimer's disease (AD) mouse models, multiphoton microscopy has opened a new window to study the real-time appearance and growth of amyloid plaques. Here, we report an alternative technology-optical-resolution photoacoustic microscopy (OR-PAM)-for in vivo imaging of amyloid plaques in a transgenic AD mouse model. In vivo validation using multiphoton microscopy shows that OR-PAM has sufficient sensitivity and spatial resolution to identify amyloid plaques in living brains. In addition, with dual-wavelength OR-PAM, the three-dimensional morphology of amyloid plaques and the surrounding microvasculature are imaged simultaneously through a cranial window. In vivo transcranial OR-PAM imaging of amyloid plaques is highly likely once the imaging parameters are optimized.

Additional Information

© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was sponsored by National Institutes of Health grants R01 NS48283, R01 NS67905, P01 NS32636 (J ML), R01 EB000712, R01 NS46214, R01 EB008085, and U54 CA136398. LVW has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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