Supplementary information
Photoacoustic
imaging
reveals
transient
decrease
of
oxygenation
in
murine
blood
due
to
monoclonal
IgG4
antibody
Anjul Khadria
1
, Chad D. Paavola
2
, Konstantin Maslov
1
, Patricia L. Brown
-
Augsburger
2
,
Patrick F. Grealish
2
,
Emmanuel Lozano
2
, Ross L. Blankenship
2
, Rui Cao
1
, Junhui Shi
1
, John
M. Beals
3
*, Sunday S Oladipupo
2
*, Lihong V. Wang
1,4
*
1
Caltech
Optical
Imaging
Laboratory,
Andrew
and
Peggy
Cherng
Department
of
Medical
Engineering,
California
Institute
of
Technology,
Pa
sadena,
California,
91125,
USA
2
Lilly
Research
Laboratories,
Eli
Lilly
and
Company,
Lilly
Corporate
Center,
Indianapolis,
Indiana,
46285,
USA
3
Lilly
Research
Laboratories,
Eli
Lilly
and
Company,
Lilly
Biotechnology
Center,
San
Diego,
California,
92121,
US
A
4
Caltech
Optical
Imaging
Laboratory,
Department
of
Electrical
Engineering,
California
Institute
of
Technology,
Pasadena,
California,
91125,
USA
Figure S
1
:
Quntification.
(a) Pharmacokinetics data of unlabeled IgG
4 isotype control
antibody
in mice
following IV and
subcutaneous
administration in
the torso or ear
; d
ata represent mean ± standard deviation.
(
b
,
c
) Photoacoustic quantification of absorption of
dye
-
labeled
IgG
4
antibody for 24 hours and 6 hours
. (d)
Photoacoustic
quantification of sulfo
-
cy7.5 dye dissolved in PBS buffer
; d
ata represent mean ± standard error
of the mean
.
a
b
c
d
Table
S1
:
Mean p
harmacokinetic parameters of unlabeled IgG
4
antibody
t
1/2
(h)
T
max
(h)
C
max
(ng/mL)
AUC
0
-
168
(h*
μ
g/mL)
AUC
all
(h*
μ
g/mL)
AUC
INF_obs
(h*
μ
g/mL)
Cl_obs
(mL/h/kg)
IV
-
tail
304
1
2351
195
414
629
0.26
SC
-
torso
520
96
1179
160
408
867
0.20
SC
-
ear
511
48
1355
197
330
944
0.19
Table
S2
:
Mean p
harmacokinetic parameters of
sulfo
-
cy7.5
dye
-
labeled IgG
4
antibody
t
1/2
(h)
T
max
(h)
C
max
(ng/mL)
AUC
0
-
168
(h*
μ
g/mL)
AUC
all
(h*
μ
g/mL)
AUC
INF_obs
(h*
μ
g/mL)
Cl_obs
(mL/h/kg)
IV
-
tail
238
1
2233
145
288
379
0.4
0
SC
-
torso
117
36
1206
130
158
235
0.70
SC
-
ear
444
12
1808
199
405
1005
0.3
0
Figure S
2
: Functional changes caused by the antibody.
(a) No significant change cuased by the dye
-
labeled antibody in
the oxygen extraction factor
with respect to the preinjection value
. Number of mice
, n
= 5
;
data represent mean ± standard deviation. All p values
at the mentioned time points were calculated
using paired t
-
test with respect to the pre
-
injection time point; p > 0.05
,
ns. (b) No change of blood sO
2
in
mouse ear after being kept under anesthesia for 5 hours continuously. (c) Blood sO
2
in the mouse ear
decreases after injection of unlabeled IgG
4
antibody. Scale bar
s
,
500 μm.
a
b
c