Self-Assembly of Elastin–Mimetic Double Hydrophobic Polypeptides
Abstract
We have constructed a novel class of "double-hydrophobic" block polypeptides based on the hydrophobic domains found in native elastin, an extracellular matrix protein responsible for the elasticity and resilience of tissues. The block polypeptides comprise proline-rich poly(VPGXG) and glycine-rich poly(VGGVG), both of which dehydrate at higher temperature but form distinct secondary structures, β-turn and β-sheet respectively. In water at 45 °C, the block polypeptides initially assemble into nanoparticles rich in β-turn structures, which further connect into long (>10 μm), beaded nanofibers along with the increase in the β-sheet content. The nanofibers obtained are well-dispersed in water, and show thermoresponsive properties. Polypeptides comprising each block component assemble into different morphologies, showing that the conjugation of poly(VPGXG) and poly(VGGVG) plays a role for beaded fiber formation. These results may provide innovative ideas for designing peptide-based materials but also opportunities for developing novel materials useful for tissue engineering and drug delivery systems.
Additional Information
© 2013 American Chemical Society. Received: December 10, 2012; Revised: February 27, 2013; Published: March 18, 2013. This work was supported by a Grant-in-Aid for Scientific Research (No. 22107005) on the Innovative Areas: "Fusion Materials" (Area No. 2206) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. D.H.T.L. is grateful to a Grant-in-Aid for Young Scientists from the Japan Society for the Promotion of Science (JSPS). A part of this work was conducted at the Center for Nanobio Integration (CNBI), Center for Medical System Integration (CMSI), and Center for Nano Lithography and Analysis, The University of Tokyo, supported by MEXT. We thank Profs. Yukio Yamaguchi and Teruyuki Nagamune for their help in the preparation of polypeptides.Attached Files
Published - bm301887m.pdf
Supplemental Material - bm301887m_si_001.pdf
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Additional details
- Eprint ID
- 38561
- Resolver ID
- CaltechAUTHORS:20130517-131452178
- 22107005
- Ministry of Education, Culture, Sports, Science and Technology (MEXT)
- Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Young Scientists
- Created
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2013-05-20Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field