Magnetic resonance microscopy of embryonic cell lineages and movements
Key events in vertebrate embryogenesis are difficult to observe in many species. High-resolution magnetic resonance imaging was used to follow cell movements and lineages in developing frog embryos. A single cell was injected at the 16-cell stage with a contrast agent, based on the gadolinium chelate gadolinium-diethylenetriamine pentaacetic acid-dextran. The labeled progeny cells could be followed uniquely in three-dimensional magnetic resonance images, acquired from the embryo over several days. The results show that external ectodermal and internal mesodermal tissues extend at different rates during amphibian gastrulation and neurulation.
© 1994 American Association for the Advancement of Science. 27 August 1993; Accepted 24 November 1993. We thank Z. H. Cho, C. B. Ahn, and S. Juh for assistance in the initial phases of this project; and J. Shih, A. Collazo, and J. Ivins for comments on the manuscript. Supported by National Institutes of Health grant HD 25390, the Baxter Foundation, and a gift from the Monsanto-Searle Company.