Progress toward an Antibody Glycosidase
The piperidine-based hapten 1 generates monoclonal antibodies that catalyze the hydrolysis of substrate 2 at pH5.5 (k_(cal) = 0.904 h^(−1), K_M = 324 μM). Hapten 1 is a competitive inhibitor of the antibody-catalyzed reaction. These results are significant for the development of antibodies for the sequence-specific hydrolysis of oligosaccharides.
© 1994 VCH Verlagsgesellschaft mbH. Received September 20, 1993. This work was supported by the Office of Naval Research (Grant No. N00014-91-J-I 130) and the Assistant Secretary for Conservation and Renewable Energy, Advanced Industrial Concepts Division of the U.S. Department of Energy (Contract No. DE-AC03-76SF00098). L C. H. is supported by a National Science Foundation Graduate Fellowship. We thank Holly C. Wessling for her assistance.