of 3
Architecture and host interface of environmental
chlamydiae revealed by electron cryotomography
Martin Pilhofer, Karin Aistleitner, Mark S. Ladinsky,
Lena König, Matthias Horn, Grant J.
Jensen
Supplementary Informatio
n
Figure S
1.
Sim
kania
,
Par
achlamydia
and
Pr
otochlamydia
do not recruit mitochondria to
their inclusions.
MitoTr
acker staining of mitochondria (red) was combined with detection of chlamydiae
(green) and the amoeba host (blue) by fluorescence
in situ
hybridization. No significant
clustering of mitochondria around inclusions of
Sim
kania
(A),
Par
achlamydia
(B) or
Pro
tochlamydia
(C) was detected in
A. castellanii
. Bars 10 μm.
Figure S
2. More examples of putative
Simkania
T3S structures with sheared
-off needles.
Shown are slices through cryotomograms of purified
Simkania
.
Often, a widening of the
perip
lasm and basal body
-like densities were observed in the absence of a T3S needle,
suggesting some needles are sheared off during purification. Bar 100 nm.
Movie S1. Crescent bodies are not seen in projection images of
plunge
-frozen
Parachlamydia
cel
ls.
Movie S2. Cryotomogram
and 3D model of the
Simkania
inclusion from Figure 3D.
Movie S3. Cryotomogram
and 3D model of the
Parachlamydia
inc
lusion from Figure 3
L.
Movie S4. Cryotomogram
and 3D model of the
Protochlamydia
inclusion from Figure
4C.
Figure S1
Sim
Par
Pro
Figure S2
A
B
C