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Published March 1, 2002 | Supplemental Material
Journal Article Open

A Genomic Regulatory Network for Development

Abstract

Development of the body plan is controlled by large networks of regulatory genes. A gene regulatory network that controls the specification of endoderm and mesoderm in the sea urchin embryo is summarized here. The network was derived from large-scale perturbation analyses, in combination with computational methodologies, genomic data, cis-regulatory analysis, and molecular embryology. The network contains over 40 genes at present, and each node can be directly verified at the DNA sequence level by cis-regulatory analysis. Its architecture reveals specific and general aspects of development, such as how given cells generate their ordained fates in the embryo and why the process moves inexorably forward in developmental time.

Additional Information

© 2002 American Association for the Advancement of Science. We are particularly grateful to E. Branscombe, who arranged for the U.S. Department of Energy's Joint Genome Institute to provide many of the BAC sequences included in this work (19); the remainder were obtained at the Institute for Systems Biology, Seattle, WA. We thank E. Rothenberg of Caltech for perspicacious and very helpful comments on the manuscript. The major embryological aspects of this work were supported by grants from NIH (HD-37105 and RR-06591); the computational aspects were supported by a grant from NIH (GM-61005); the comparative aspects were supported by a grant from the NASA/Ames Fundamental Space Biology program (NAG2-1368); the arrayed cDNA and BAC libraries on which the project depended were produced with the support of a grant from NIH (RR-15044); and the work on the brachyury gene was supported by a Human Frontiers grant (RG0290) to R.A.C. Other support was provided by the Lucille P. Markey Charitable Trust, the Stowers Institute for Medical Research, the Beckman Institute of Caltech, and a grant from NSF (IBN99882875) to R.A.C.

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