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Published April 2013 | public
Conference Paper

Selective oligonucleotide and mRNA pull-down with shielded covalent probes


Shielded covalent (SC) probes combine programmable base pairing, mol. conformation change, and activatable covalent crosslinking to achieve selective and durable capture of nucleic acid targets, including efficient discrimination of SNPs. Capture yields appear consistent with the thermodn. of probe/target hybridization, allowing rational probe design. We will demonstrate RNA pull-down using surface-immobilized SC probes, exploiting covalent target capture to remove unwanted material using stringent washes, and then reversing the crosslinks to recover the targets. RNA pull-downs using SC probes will provide a powerful framework for exploring the in vivo binding partners of RNAs.

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© 2013 American Chemical Society.

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August 19, 2023
October 24, 2023