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Published August 1942 | Published
Journal Article Open

The manufacture of antibodies in vitro


A protein solution with the properties of a specific antiserum to the triphenylmethane dye methyl blue has been made by treating a solution of bovine gamma-globulin and the dye with alkali and then slowly neutralizing the alkali. Some success has been obtained also in the formation of antibodies from other serum proteins and by other denaturation-renaturation procedures. By heating solutions of gamma-globulin and antigen to 57°C. for several days antisera homologous to the antigens have been prepared. This method has been used successfully with the azodye 1,3-dihydroxy-2,4,6-tri(p-azophenyl-arsonic acid) benzene and with pneumococcus polysaccharide Type III. The antipneumococcus sera were found to precipitate the polysaccharide of Type III but not those of Types I and VIII and to agglutinate pneumococci of Type III but not those of Types I and II.

Additional Information

© 1942, by The Rockefeller Institute for Medical Research New York. (Received for publication, April 25, 1942) A brief statement of the results of these experiments has beea published: Pauling, L., and Campbell, D. H., Science, 1942, 95, 440. We acknowledge with appreciation the financial support of this work by the Rockefeller Foundation. We are also indebted to Dr. David Pressman and Mr. Carol Ikeda of these Laboratories for help with the experiments, and to Dr. W. Goebel of The Rockefeller Institute for Medical Research, Dr. E. J. Cohn of Harvard Medical School, and Dr. J. D. Porsche of Armour and Co. for providing us with materials.

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