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nature research | life sciences reporting summary
November 2017
Corresponding author(s):
Kevin McDonnell
Life Sciences Reporting Summary
Nature Research wishes to improve the reproducibility of the work that we publish. This form is intended for publication with a
ll accepted life
science papers and provides structure for consistency and transparency in reporting. Every life science submission will use thi
s form; some list
items might not apply to an individual manuscript, but all fields must be completed for clarity.
For further information on the points included in this form, see
Reporting Life Sciences Research
. For further information on Nature Research
policies, including our
data availability policy
, see
Authors & Referees
and the
Editorial Policy Checklist
.
Please do not complete any field with "not applicable" or n/a. Refer to the help text for what text to use if an item is not r
elevant to your study.
For final submission: please carefully check your responses for accuracy; you will not be able to make changes later.
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Experimental design
1. Sample size
Describe how sample size was determined.
Minimal sample sizes were determined to ensure generation of statistical values for the
experiments, in line with previously published studies employing these techniques.
2. Data exclusions
Describe any data exclusions.
No data was excluded from study
3. Replication
Describe the measures taken to verify the reproducibility
of the experimental findings.
All replication attempts were successful. Methods sections provides detailed desciptions so
that other laboratories may successfully repeat experiments
4. Randomization
Describe how samples/organisms/participants were
allocated into experimental groups.
Current study is not a randomized control trial and randomization not conventionally
performed for in vitro experiments in this study.
5. Blinding
Describe whether the investigators were blinded to
group allocation during data collection and/or analysis.
Current study is not a blinded trial and blinding not conventionally performed for in vitro
experiments in this study.
Note: all in vivo studies must report how sample size was determined and whether blinding and randomization were used.
6. Statistical parameters
For all figures and tables that use statistical methods, confirm that the following items are present in relevant figure legend
s (or in the
Methods section if additional space is needed).
n/a
Confirmed
The exact sample size (
n
) for each experimental group/condition, given as a discrete number and unit of measurement (animals, litters, cultures, etc.)
A description of how samples were collected, noting whether measurements were taken from distinct samples or whether the same
sample was measured repeatedly
A statement indicating how many times each experiment was replicated
The statistical test(s) used and whether they are one- or two-sided
Only common tests should be described solely by name; describe
more complex techniques in the Methods section.
A description of any assumptions or corrections, such as an adjustment for multiple comparisons
Test values indicating whether an effect is present
Provide confidence intervals or give results of significance te
sts (e.g. P values) as exact values whenever appropriate and wi
th effect sizes noted.
A clear description of statistics including central tendency (e.g. median, mean) and variation (e.g. standard deviation, interq
uartile range)
Clearly defined error bars in all relevant figure captions (with explicit mention of central tendency and variation)
See the web collection on
statistics for biologists
for further resources and guidance.
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nature research | life sciences reporting summary
November 2017
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Software
Policy information about
availability of computer code
7. Software
Describe the software used to analyze the data in this
study.
Prism GraphPad
For manuscripts utilizing custom algorithms or software that are central to the paper but not yet described in the published li
terature, software must be made
available to editors and reviewers upon request. We strongly encourage code deposition in a community repository (e.g. GitHub).
Nature Methods
guidance for
providing algorithms and software for publication
provides further information on this topic.
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Materials and reagents
Policy information about
availability of materials
8. Materials availability
Indicate whether there are restrictions on availability of
unique materials or if these materials are only available
for distribution by a third party.
Reagents are available from commercial sources.
9. Antibodies
Describe the antibodies used and how they were validated
for use in the system under study (i.e. assay and species).
No antibodies were used in these experiments.
10. Eukaryotic cell lines
a. State the source of each eukaryotic cell line used.
No eukaryotic cell lines were used in these experiments.
b. Describe the method of cell line authentication used.
No eukaryotic cell lines were used in these experiments.
c. Report whether the cell lines were tested for
mycoplasma contamination.
No cell lines were used in these experiments.
d. If any of the cell lines used are listed in the database
of commonly misidentified cell lines maintained by
ICLAC
, provide a scientific rationale for their use.
No commonly misidentified cell lines were used in these experiments.
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Animals and human research participants
Policy information about
studies involving animals
; when reporting animal research, follow the
ARRIVE guidelines
11. Description of research animals
Provide all relevant details on animals and/or
animal-derived materials used in the study.
No animals were used in these experiments.
Policy information about
studies involving human research participants
12. Description of human research participants
Describe the covariate-relevant population
characteristics of the human research participants.
Patient made reference to in current study is enrolled in an IRB-approved cancer genetics
registry.