Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published March 2012 | Accepted Version
Journal Article Open

Multimodal sentinel lymph node mapping with single-photon emission computed tomography (SPECT)/computed tomography (CT) and photoacoustic tomography


The identification of cancer cells in the lymph nodes surrounding a tumor is important in establishing a prognosis. Optical detection techniques such as fluorescence and photoacoustic tomography (PAT) have been reported in preclinical studies for noninvasive sentinel lymph node (SLN) mapping. A method for validation of these techniques is needed for clinical trials. We report the use of a multimodal optical-radionuclear contrast agent as a validation tool for PAT in a preclinical model. Methylene blue (MB) was radiolabeled with ^(125)I for multimodal SLN mapping and used in conjunction with MB to assess the feasibility of multimodal SLN mapping in a rat model by PAT and single-photon emission computed tomography (SPECT). MB provided sufficient contrast for identifying SLNs noninvasively with a PAT system adapted from a clinical ultrasound imaging system. The signal location was corroborated by SPECT using ^(125)I labeled MB. The translation of PAT into the clinic can be facilitated by a direct comparison with established imaging methods using a clinically relevant dual SPECT and photoacoustic imaging agent. The new high-resolution PAT is a promising technology for the sensitive and accurate SLN detection in cancer patients.

Additional Information

© 2012 Mosby, Inc. Received 4 May 2011, Revised 8 August 2011, Accepted 27 September 2011, Available online 21 October 2011. Supported in part by grants R01 EB008458, R01 EB007276, R01 EB008111, R01 EB000712, R01 EB008085, R33 CA123537, and U54 CA136398 from the National Institutes of Health (the Network for Translational Research) and by Grant K01RR026095 from the National Center for Research Resources (to W. J. A.).

Attached Files

Accepted Version - nihms354047.pdf


Files (2.5 MB)
Name Size Download all
2.5 MB Preview Download

Additional details

August 19, 2023
October 19, 2023