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Published December 22, 2004 | Supplemental Material
Journal Article Open

Design and characterization of programmable DNA nanotubes


DNA self-assembly provides a programmable bottom-up approach for the synthesis of complex structures from nanoscale components. Although nanotubes are a fundamental form encountered in tile-based DNA self-assembly, the factors governing tube structure remain poorly understood. Here we report and characterize a new type of nanotube made from DNA double-crossover molecules (DAE-E tiles). Unmodified tubes range from 7 to 20 nm in diameter (4 to 10 tiles in circumference), grow as long as 50 μm with a persistence length of ~4 μm, and can be programmed to display a variety of patterns. A survey of modifications (1) confirms the importance of sticky-end stacking, (2) confirms the identity of the inside and outside faces of the tubes, and (3) identifies features of the tiles that profoundly affect the size and morphology of the tubes. Supported by these results, nanotube structure is explained by a simple model based on the geometry and energetics of B-form DNA.

Additional Information

© 2004 American Chemical Society. Received September 17, 2004. Publication Date (Web): November 24, 2004. For useful discussions we thank Matthew Cook and Hideo Mabuchi. We thank the Caltech Molecular Materials Research Center for use of their AFM scanners. P.W.K.R. was supported by a Beckman Fellowship. This work was supported in part by National Science Foundation CAREER Grant No. 0093486 and MRSEC Award DMR00-80034, DARPA BioComputation Contract F30602-01-2-0561, NASA NRA2-37143, an Army Research Office/UCSB Institute for Collaborative Biotechnologies Grant, an Alfred P. Sloan Foundation Fellowship (D.K.F.), and a grant from GenTel Corporation.

Attached Files

Supplemental Material - ja044319lsi20040917_035440.mpg

Supplemental Material - ja044319lsi20040917_035521.mpg

Supplemental Material - ja044319lsi20040917_035648.mpg

Supplemental Material - ja044319lsi20041022_031528.pdf


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