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Published July 15, 2013 | Published
Journal Article Open

Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth


Due to strong light scattering in tissue, both the spatial resolution and maximum penetration depth of optical-resolution photoacoustic microscopy (OR-PAM) deteriorate sharply with depth. To reduce tissue scattering, we propose to use glycerol as an optical clearing agent in OR-PAM. Our results show that the imaging performance of OR-PAM can be greatly enhanced by optical clearing both in vitro and in vivo.

Additional Information

© 2013 Optical Society of America. Received June 3, 2013; accepted June 13, 2013; posted June 25, 2013 (Doc. ID 191645); published July 15, 2013. The authors would like to thank Prof. James Ballard for manuscript editing. We also appreciate technical assistance and useful discussions from Jinyang Liang and Chi Zhang. This research was supported by the National Institutes of Health Grants DP1 EB016986 (NIH Director's Pioneer Award), R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959. L. V. W. has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work.

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