Phosphorylation of Synaptic GTPase-activating Protein (synGAP) by Polo-Like Kinase (Plk2) alters the Ratio of Its GAP Activity toward HRas, Rap1 and Rap2 GTPases

Phosphorylation of Synaptic GTPase-activating Protein

(synGAP) by Polo-Like Kinase (Plk2) alters the Ratio of Its GAP

Activity toward HRas, Rap1 and Rap2 GTPases

Ward G. Walkup 4th

a,b,*

Michael J. Sweredoski

Robert L. Graham

c,d

Sonja Hess

c,e

Mary

B. Kennedy

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA

91125

Current affiliation: De Novo Discovery, FogPharma, Cambridge, MA

Proteome Exploration Laboratory of the Beckman Institute, California Institute of Technology,

Pasadena, CA 91125

Current affiliation: Institute of Cancer Sciences, Faculty of Medical and Human Sciences,

University of Manchester, UK M13 9PL

Current affiliation: Antibody Discovery and Protein Engineering, MedImmune, Gaithersburg, MD

Abstract

SynGAP is a Ras and Rap GTPase-activating protein (GAP) found in high concentration in the

postsynaptic density (PSD) fraction from mammalian forebrain where it binds to PDZ domains of

PSD-95. Phosphorylation of pure recombinant synGAP by Ca

/calmodulin-dependent protein

kinase II (CaMKII) shifts the balance of synGAP’s GAP activity toward inactivation of Rap1;

whereas phosphorylation by cyclin-dependent kinase 5 (CDK5) has the opposite effect, shifting

the balance toward inactivation of HRas. These shifts in balance contribute to regulation of the

numbers of surface AMPA receptors, which rise during synaptic potentiation (CaMKII) and fall

during synaptic scaling (CDK5). Polo-like kinase 2 (Plk2/SNK), like CDK5, contributes to

synaptic scaling. These two kinases act in concert to reduce the number of surface AMPA

receptors following elevated neuronal activity by tagging

spine-

associated

Rap-specific GAP

protein (SPAR) for degradation, thus raising the level of activated Rap. Here we show that Plk2

also phosphorylates and regulates synGAP. Phosphorylation of synGAP by Plk2 stimulates its

GAP activity toward HRas by 65%, and toward Rap1 by 16%. Simultaneous phosphorylation of

synGAP by Plk2 and CDK5 at distinct sites produces an additive increase in GAP activity toward

HRas (~230%) and a smaller, non-additive increase in activity toward Rap1 (~15%). Dual

Corresponding author. Division of Biology and Biological Engineering 216-76, California Institute of Technology, Pasadena, CA,

USA 91125, wwalkup@alumni.caltech.edu (W.G. Walkup 4th).

Appendix A. Supplementary Data

Supplementary data related to this article is attached as a separate document.

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Author manuscript

Biochem Biophys Res Commun

. Author manuscript; available in PMC 2021 February 19.

Published in final edited form as:

Biochem Biophys Res Commun

. 2018 September 10; 503(3): 1599–1604. doi:10.1016/

j.bbrc.2018.07.087.

Author Manuscript

phosphorylation also produces an increase in GAP activity toward Rap2 (~40–50%), an effect not

produced by either kinase alone. As we previously observed for CDK5, addition of Ca

/CaM

causes a substrate-directed doubling of the rate and stoichiometry of phosphorylation of synGAP

by Plk2, targeting residues also phosphorylated by CaMKII. In summary, phosphorylation by

Plk2, like CDK5, shifts the ratio of GAP activity of synGAP to produce a greater decrease in

active Ras than in active Rap, which would produce a shift toward a decrease in the number of

surface AMPA receptors in neuronal dendrites.

Keywords

synaptic plasticity; synaptic scaling; postsynaptic density; synaptic GTPase activating protein

(synGAP); cyclin-dependent kinase 5 (CDK5); polo-like kinase 2 (Plk2)

1. Introduction

The post synaptic density (PSD) of neurons harbors several cytosolic signaling complexes

that are associated with glutamate receptors at the postsynaptic membrane of excitatory

synapses [

]. synGAP, a dual Ras and Rap GTPase Activating Protein (GAP), is unusually

highly concentrated in the PSD of excitatory synapses [

–

]. It binds tightly to the PDZ

domains of PSD-95 [

] which serves to position it in close proximity to NMDA-type and

AMPA-type glutamate receptors (NMDARs and AMPARs, respectively), and allows it to

regulate the composition of the PSD by restricting binding of other proteins to PSD-95 [

SynGAP stimulates the intrinsic GTPase activity of Rap ~100-fold and that of Ras, which

has a higher intrinsic rate, about 3 to 7-fold [

]. Stimulation of Ras and Rap by various

signaling pathways [

–

] modulates AMPAR trafficking in opposite directions, with active

Ras increasing insertion (exocytosis) of AMPARs at the dendritic membrane, while active

Rap increases their removal (endocytosis) [

Activation of NMDARs in cultured CNS neurons leads to phosphorylation of synGAP by

CaMKII [

]. We showed that a recombinant, purified, soluble synGAP-

1 that lacks

102 residues at the N terminus (r-synGAP) can be expressed in soluble form and

phosphorylated by purified Ca

/calmodulin-dependent protein kinase II (CaMKII) and

cyclin-dependent kinase 5 (CDK5) [

]. Phosphorylation by CaMKII accelerates the rate of

inactivation of Rap1 more potently than the rate of inactivation of HRas, whereas

phosphorylation by CDK5 has the opposite effect [

Within postsynaptic spines, Spine-associated RapGAP (SPAR), also regulates Rap [

SPAR acts differently than synGAP because it stimulates the activity of Rap2 more potently

than Rap1. CDK5 and polo-like kinase 2 (Plk2/SNK) act in concert to regulate SPAR;

CDK5 primes phosphorylation by Plk2, ultimately tagging SPAR for degradation [

During homeostatic down-regulation of synapses, loss of SPAR activity increases the steady-

state level of active Rap, thus increasing endocytosis of surface AMPA receptors.

We previously showed that Plk2 phosphorylation of r-synGAP decreases its affinity for

PSD-95 [

] and that CDK5 phosphorylation of r-synGAP accelerates its HRas GAP activity

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[

]. Here we extend those studies by examining the effect of phosphorylation by Plk2 on

GAP activity of r-synGAP and the effect of simultaneous phosphorylation of r-synGAP by

Plk2 and CDK5. We determine the location and stoichiometry of Plk2 phosphorylation sites

in r-synGAP, and the effect of their phosphorylation on HRas and Rap1/Rap2 GAP activity.

We show that, as for CDK5, the addition of Ca

/CaM produces a substrate-directed

increase in phosphorylation. Finally, we show that simultaneous phosphorylation by CDK5

and Plk2 results in a large additive increase in GAP activity toward HRas and a much

smaller increase in GAP activity toward both Rap1 and Rap2.

2. Materials and Methods

2.1 Expression and Purification of Recombinant Proteins.

R-synGAP, residues 103–1293 in synGAP A1

1 (118–1308 in synGAP A2-

1), and full

length HRas, Rap1B and Rap2A were purified as previously described [

]. We used synGAP

isoform names and residue numbering from ref. [

] with all residue numbering

corresponding to synGAP A1-

2.2 Stoichiometry and Rate of R-synGAP or

-casein Phosphorylation by Plk2.

R-synGAP (286 nM) or dephosphorylated

-casein (3.7 μM) from bovine milk (Sigma-

Aldrich) was phosphorylated with 110 nM Plk2 (Life Technologies). Phosphorylated

proteins were detected and calculation of the stoichiometry of phosphorylation was

performed as described in [

2.3 Phosphorylation of R-synGAP by Plk2 or CDK5 for use in GTPase Assays.

Phosphorylation of 725 nM r-synGAP by 230 nM Plk2, 230 nM CDK5/p35 or 230 nM Plk2

and CDK5/p35 was carried out as described in [

]. GTPase assays were carried out under

conditions previously described in [

2.4 Mass Spectrometry of Phosphorylated R-synGAP.

Mass spectrometry of phosphorylated r-synGAP using a hybrid LTQ-FT (Thermo Scientific)

equipped with a nano-electrospray ion source (Thermo Scientific) was carried out by the

Proteome Exploration Laboratory at the California Institute of Technology as previously

described [

3. Results and Discussion

3.1 Stoichiometry and Rate of Phosphorylation of r-SynGAP by Plk2.

Both endogenous and recombinant synGAP expressed in COS7 cells [

] can be

phosphorylated by Plk2. In our assay, r-synGAP was phosphorylated by Plk2 (Fig. 1A) at a

rate and stoichiometry similar to that of CDK5 phosphorylation [

]. The reaction was linear

for 10 min, reaching a stoichiometry of ~0.4 mol phosphate/mol r-synGAP. After 30 min,

the stoichiometry approached ~0.8 mol phosphate/mol. As for CDK5, addition of Ca

/CaM

to Plk2 phosphorylation reactions doubles the stoichiometry and rate of r-synGAP

phosphorylation to ~0.8 and ~1.8 mol phosphate/mol at 10 and 30 minutes, respectively

(Fig. 1A). In contrast, phosphorylation by Plk2 of dephosphorylated

⍺

-caesin, was

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unaffected by Ca

/CaM (Fig. 1B). We previously showed that synGAP contains a binding

site for Ca

/CaM with affinity in the nM range [

]. Thus, the simplest explanation for the

effect of Ca

/CaM is that its binding to synGAP induces a conformational change that

increases accessibility to CDK5 and Plk2, accelerating the kinase reaction rate and enabling

the phosphorylation of additional residues.

3.2 Identification of Sites in r-SynGAP Phosphorylated by Plk2.

Recombinant synGAP expressed in COS cells was phosphorylated at nine residues by Plk2

(S364, S370, S434, S451, S821, S825, S827 and S880); however, phosphorylation at only

three of them (S370, S434, S825 and S827) influenced synGAP’s HRas GAP activity [

We identified eight additional Plk2 phosphorylation sites in r-synGAP (S140, S750, S751,

S756, S765, S808, S810, T897), and confirmed phosphorylation at S821, S825 and S827

vitro

(Table 1, Supplemental Table 1 and Data). All of the identified Plk2 phosphorylation

sites had high Mascot scores and false localization rates of less than 1%. Phosphorylation at

sites S140, S750, S751, S756, S765, S808 was detected after 2 minutes of reaction with

Plk2, whereas phosphorylation at S810, S821, S825, S827, and T897 was only detectable

after 10 minutes of reaction with Plk2. We previously showed that addition of Ca

/CaM to

reactions with CDK5/p35 results in phosphorylation of r-synGAP at S751, S765, S810,

S1093 and S1123, in addition to sites S728, S773/T775, S802 and S842 [

] (Table 2,

Supplemental Table 2 and Data). Similarly, here we show that addition of Ca

/CaM results

in phosphorylation of S810 and T897 by Plk2 within 2 min, and phosphorylation of S1099,

S1123 and S1283, which are also phosphorylated by CaMKII, after 10 min [

] (Table 1,

Supplemental Table 1 and Data). Plk2 and Plk3 kinases prefer phosphorylation sites in

which acidic amino acids (D, E) occur at positions between −4 and +4 of the target serine or

threonine residue; in endogenous substrates, this preference is often extended to additional

positions (e.g. −7 to +7) [

–

]. All of the Plk2 phosphorylation sites in r-synGAP, except

S1123, contain one (S140, S756, S765, S808, S821, S825, S827, T897, S1099, S1283), two

(S750, S751) or three (S810) acidic residues within −6 to +6 residues of the target serine or

threonine residue.

3.3 Effect of Plk2 Phosphorylation on GAP Activity of R-synGAP.

Plk2 phosphorylation increases the RasGAP activity of synGAP in COS cell lysates [

We examined the effect of phosphorylation of r-synGAP on its GAP activity toward Rap1,

Rap2, and Ras, using the

in vitro

Ras and Rap GTPase assays described in [

]. As we

observed for CDK5, phosphorylation by Plk2 increased the HRas GAP activity of r-synGAP

substantially (70%) (Fig. 2A), and the Rap1 GAP activity slightly (15%) (Fig. 2B), but did

not affect GAP activity of Rap2 (Fig. 2C). The effect of Plk2 on HRas GAP activity was

significant after 1 min and continued to rise throughout the time course, whereas the effect

on Rap1 was nearly maximal (11%) after 2 min. Addition of Ca

/CaM to phosphorylation

reactions did not alter HRas or Rap1 GAP activity significantly, but caused a small increase

in Rap2 GAP activity (11%) after 10 min. These data show that phosphorylation of r-

synGAP by Plk2 accelerates its rate of inactivation of HRas considerably more potently than

inactivation of Rap1 or Rap2, which

in vivo

would result in a relative increase in the steady-

state level of active Rap, thus increasing endocytosis of surface AMPA receptors [

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3.4 Effect of Concomitant CDK5 and Plk2 Phosphorylation on GAP Activity of R-synGAP.

During synaptic scaling, Plk2 binds to and phosphorylates SPAR that has previously been

phosphorylated at a specific site by CDK5. The phosphorylation targets SPAR for

ubiquitination, ultimately leading to a loss of mature spines and excitatory synapses [

Efficient ubiquitination of SPAR requires the “priming” phosphorylation by CDK5 [

]. We

found that concomitant phosphorylation of r-synGAP by Plk2 and CDK5 increases its GAP

activity toward HRas substantially and synergystically (230%, Fig. 3A). It had only a slight

effect on GAP activity toward Rap1 (18%, Fig. 3B), but produced a substantial increase in

GAP activity toward Rap2A that appeared more than additive (40%, Fig. 3C). Addition of

/CaM to these reactions had no statistically significant effects on GAP activity toward

HRas (Fig. 3A) or Rap1 (Fig. 3B) and a slight effect on GAP activity toward Rap2 (Fig. 3C).

These data suggest that Plk2 and CDK5 act together to drive the synapse toward removal of

AMPARs through activation of the Rap pathway by triggering removal of SPAR, and by

increasing the GAP activity of r-synGAP toward HRas. Activation of Plk2 also triggers

elimination of the HRas activator RasGRF1 and stimulates the Rap activator PDZGEF1

[

3.5 CaMKII, CDK5, and Plk2 Produce a Continuum of Changes in the Relative Levels of

Ras and Rap GAP Activity of synGAP.

Ras and Rap are critical regulators of the endocytosis and exocytosis of AMPARs near the

synapse [

]. The steady-state levels of activity of these two GTPases are set by the balance

of their activation by exchange of GDP for GTP and their inactivation by hydrolysis of the

bound GTP. Phosphorylation of synGAP by CaMKII alters the ratio of inactivation of Rap1

and HRas (Fig. 4A) [

] in a direction that would shift the steady-state balance of AMPAR

trafficking toward exocytosis resulting in an increase in surface AMPA receptors.

Conversely, phosphorylation of synGAP by CDK5 or Plk2 alters the ratio in the opposite

direction (Fig. 4A) [

], shifting the balance of AMPAR trafficking toward endocytosis of

AMPA receptors and a decrease in the number of surface AMPARs. Concomitant

phosphorylation by Plk2 and CDK5 drives up the rate of inactivation of HRas by synGAP

even further (Fig. 4A), which would strongly drive the endocytosis of AMPARs. CaMKII is

activated rapidly when Ca

flows through NMDA receptors during induction of LTP [

];

whereas both Plk2 and CDK5 are induced by activity-driven protein synthesis over a longer

time scale [

]. CDK5 activity is further limited by the availability of its p35 and p39

subunits, which are constitutively degraded and require NGF or BDNF to stimulate synthesis

[

]. The absence of rapid activation mechanisms and the slow catalytic rates of CDK5 and

Plk2 and are consistent with a homeostatic role. Thus, phosphorylation of synGAP by

combinations of different kinases could function like a rheostat to rapidly or gradually adjust

the steady-state number of surface AMPA receptors at each synapse.

In addition to altering the ratio of GAP activity toward HRas or Rap1, phosphorylation of

synGAP can also alter the ratio of GAP activity toward Rap1 or Rap2 (Fig. 4B).

Phosphorylation by CaMKII increases the ratio of GAP activity toward Rap1 versus Rap2

by markedly increasing the Rap1 GAP activity. Concomitant phosphorylation of synGAP by

Plk2 and CDK5 has the opposite effect, decreasing the ratio of GAP activity toward Rap1

versus Rap2 by increasing Rap2 GAP activity. Rap1 and Rap2 are both present in pyramidal

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neurons in the hippocampus and cortex, but the differences in their functions are not yet

clear. LTD inducing stimuli stimulate the Rap1-dependent p38 MAPK pathway, resulting in

phosphorylation of AMPARs with short cytoplasmic tails (GluR2/3) and their subsequent

exocytosis [

], while the Rap2-dependent JNK pathway is activated by depotentiation-

inducing stimuli and causes dephosphorylation of AMPARs with long cytoplasmic tails

(GluR1, GluR2L) and their removal from the synapse [

]. Given the degree of crosstalk

between the Rap1, Rap2 and Ras pathways, and the diverse localization of small GTPases

and their activators and effectors, it is plausible that distinct sub cellular pools of small

GTPases may perform discrete functions in the synapse [

Supplementary Material

Refer to Web version on PubMed Central for supplementary material.

Acknowledgements

This work was supported by grants from the Gordon and Betty Moore Foundation (Center for Integrative Study of

Cell Regulation), the Hicks Foundation for Alzheimer’s Research, the Allen and Lenabelle Davis Foundation, and

from National Institutes of Health Grant MH095095 to MBK. WGW IV was supported by the National Science

Foundation Graduate Research Fellowship under Grant No. 2006019582 and National Institutes of Health under

Grant No. NIH/NRSA 5 T32 GM07616. The PEL is supported by the Gordon and Betty Moore Foundation through

grant GBMF775 and the Beckman Institute.

Abbreviations

(synGAP)

synaptic GTPase activating protein

(r-synGAP)

soluble recombinant synGAP fragment comprising

residues 103–1293

(CaM)

calmodulin

(CaMKII)

/calmodulin-dependent protein kinase II

(CDK5)

cyclin-dependent kinase 5

(Plk2)

polo-like kinase 2

(HRas)

p21 Ras

(Rap1/2)

Ras-related protein-1 or 2

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