Published June 25, 2003
| Supplemental Material
Journal Article
Open
Breaking the degeneracy of the genetic code
Abstract
A mutant yeast phenylalanine transfer RNA (ytRNA^(Phe)_(AAA)) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNA^(Phe)_(AAA) and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.
Additional Information
Copyright © 2003 American Chemical Society. Published In Issue June 25, 2003. Publication Date (Web): May 29, 2003. Received March 5, 2003. Acknowledgment. This work was supported by NIH GM62523 and by the NSF MRSEC program. We thank Dr. Mona Shahgholi, Dr. Gary M. Hathaway, and Dr. Jie Zhou for mass spectrometry studies. We thank Pin Wang and Soojin Son for helpful discussions. Supporting Information Available: Protein expression gels and LC/MS/MS spectra (PDF). Also found in: Conference: 225th National Meeting of the American-Chemical-Society Location: New Orleans, LA. Date: MAR 23-27, 2003. Sponsor(s): Amer Chem Soc. Abstracts of Papers of the American Chemical Society. Volume: 225. Pages: U322-U322. Part: 2. Meeting Abstract: 255-ORGN. Published: MAR 2003.Attached Files
Supplemental Material - ja0350076si20030504_032638.pdf
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Additional details
- Eprint ID
- 53818
- DOI
- 10.1021/ja0350076
- Resolver ID
- CaltechAUTHORS:20150116-102631008
- GM62523
- NIH
- NSF MRSEC
- Created
-
2015-01-16Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field