Mapping chain unfolding in macromolecular networks under stress

Abstract

We have prepd. well-defined macromol. networks that contain engineered fibronectin domains that unfold when subjected to mech. stress. Unfolding of the network chains can be monitored by observing changes in Forster resonance energy transfer (FRET) between pairs of dyes that flank the fibronectin domains. This lecture will describe the spatial mapping of FRET efficiency in protein hydrogels subject to non-uniform mech. stress fields.

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