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Published October 1, 1971 | public
Journal Article Open

Purification and Partial Characterization of Bacillus subtilis Flagellar Hooks


A method for preparing bacterial flagellar hook structures is described. The method involves isolating intact flagella from a mutant which makes thermally labile flagellar filaments and heat-treating them to disaggregate the filament preferentially. The resulting hook preparation can be separated and purified by velocity and isopycnic centrifugation. The purified hooks sediment at a relative S value of 77. On acrylamide gel electrophoresis in sodium dodecyl sulfate, they show one major and a number of minor protein bands. The purified hooks can be used to immunize rabbits, and the resulting antiserum is hook-specific. These results support the notion that hooks are composed of a protein that differs from flagellin.

Additional Information

Copyright © 1971 American Society for Microbiology. Received for publication 7 July 1971 This investigation was supported by grant no. GB-15655 from the National Science Foundation. Presented in part at the Xth International Congress of Microbiology, Mexico City, August 1970.


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