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Published December 28, 2007 | Accepted Version + Supplemental Material
Journal Article Open

Architecture of a Coat for the Nuclear Pore Membrane


The symmetric core of the nuclear pore complex can be considered schematically as a series of concentric cylinders. A peripheral cylinder coating the pore membrane contains the previously characterized, elongated heptamer that harbors Sec13-Nup145C in its middle section. Strikingly, Sec13-Nup145C crystallizes as a hetero-octamer in two space groups. Oligomerization of Sec13-Nup145C was confirmed biochemically. Importantly, the numerous interacting surfaces in the hetero-octamer are evolutionarily highly conserved, further underlining the physiological relevance of the oligomerization. The hetero-octamer forms a slightly curved, yet rigid rod of sufficient length to span the entire height of the proposed membrane-adjacent cylinder. In concordance with the dimensions and symmetry of the nuclear pore complex core, we suggest that the cylinder is constructed of four antiparallel rings, each ring being composed of eight heptamers arranged in a head-to-tail fashion. Our model proposes that the hetero-octamer would vertically traverse and connect the four stacked rings.

Additional Information

© 2007 Elsevier Inc. Received 27 August 2007, Revised 26 October 2007, Accepted 29 November 2007, Available online 27 December 2007. We thank E. Debler, O. Dreesen, I. Melcak, V. Nagy, J. Napetschnig, and A. Patke for discussions and comments on the manuscript, S. Etherton for help with editing the manuscript, and D. King for mass spectrometry analysis. In addition, we thank C. Ralston, B. Manjasetty, and W. Shi for support during data collection. N-terminal protein sequencing was performed by the Protein Center of the Rockefeller University. A.H. was supported by a grant from the Leukemia and Lymphoma Society. Accession Numbers: The atomic coordinates of the monoclinic and orthorhombic structures have been deposited to the Protein Data Bank with the accession codes 3BG0 and 3BG1.

Attached Files

Accepted Version - nihms36973.pdf

Supplemental Material - 1-s2.0-S0092867407015425-mmc1.pdf

Supplemental Material - 1-s2.0-S0092867407015425-mmc2.mov

Supplemental Material - 1-s2.0-S0092867407015425-mmc3.mov

Supplemental Material - 1-s2.0-S0092867407015425-mmc4.mov

Supplemental Material - 1-s2.0-S0092867407015425-mmc5.mov


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