Neural crest origin of sympathetic neurons
at the dawn of vertebrates
In the format provided by the
authors and unedited
Nature | www.nature.com/nature
Supplementary information
https://doi.org/10.1038/s41586-024-07297-0
SI Table 1: Top Molecular Function GO terms
Name
P-val
ue
Adjusted p-val
ue
Odds
Ratio
Combined sco
re
1 Protein Homodi
meriza tion
Act ivi ty (GO:0042803)
7.29E
-07
0.0003711
2.56
36.
22
2 Adenyl
Ribonucl
eot
ide
Bindi
ng
(GO:0032559)
0.00003671
0.009342
2.92
29.
87
3 ATP Bindi
ng
(GO:0005524)
0.00008307
0.01409
2.92
27.
46
4 Insu lin -Like Growth Fact or I B
indi
ng
(GO:0031994)
0.0001629
0.02072
19.
65
171.
39
5 Carboxylic
Est er Hyd rolase Act ivi ty (GO:0052689)
0.0002785
0.02759
4.21
34.
49
6 Kinase
Act ivi ty (GO:0016301)
0.0003252
0.02759
4.13
33.
13
7 Insu lin -Like Growth Fact or Bindi
ng
(GO:0005520)
0.0004231
0.03076
14.
29
110.
99
8 Carboxylic
Aci d Transm
embrane
Transpor
ter Act ivi ty (GO:0046943)
0.000649
0.04129
5.31
38.
98
9 Vascu
lar Endot
helia
l Growth Fact or Recept
or 2 Bindi
ng
(GO:0043184)
0.0007919
0.04479
23.
54
168.
07
10 Monoca
rboxylic
Aci d Transm
embrane
Transpor
ter Act ivi ty (GO:0008028)
0.001103
0.05395
7.29
49.
61
11 Amino
Aci d:Monoat
omic Cation
Sym
por
ter Act ivi ty (GO:0005416)
0.001166
0.05395
19.
61
132.
46
12 Pyr idoxa
l Phosphat
e Bindi
ng
(GO:0030170)
0.00136
0.05769
9.82
64.
82
13 Vincu lin Bindi
ng
(GO:0017166)
0.001635
0.06401
16.
81
107.
85
14 Endopept
idase
Inhi
bitor Act ivi ty (GO:0004866)
0.002004
0.07285
3.45
21.
45
15 Oxi dor
educt
ion-
Driven
Act ive Transm
embrane
Transpor
ter Act ivi ty (GO:0015453)
0.002215
0.07453
4.92
30.
09
16 Flavi n Adeni
ne
Dinucl
eot
ide
Bindi
ng
(GO:0050660)
0.002435
0.07453
4.82
29.
01
17 Oxi dor
educt
ase Act ivi ty, Act ing O
n The
CH-
OH Group Of D
onor
s, NAD Or NADP As Accept
or (GO:0016616)
0.002621
0.07453
3.63
21.
57
18 2 Iron,
2 Sulfur Clu
st er Bindi
ng
(GO:0051537)
0.002887
0.07453
13.
07
76.
44
19 Vascu
lar Endot
helia
l Growth Fact or Recept
or Bindi
ng
(GO:0005172)
0.002887
0.07453
13.
07
76.
44
20
Cadher
in Bindi
ng
(GO:0045296)
0.002928
0.07453
2.24
13.
05
21.
57
Molecular Function GO
analysis for lamprey TH
+
neurons. Analysis is performed using the top 500 significantly upregulated transcripts with an enrichment cutoff of
1.5 log
2
(Fold Change). p-values are determined using Fisher’s exact test.
SI Table 2: Top Biological Process GO terms
Name
P-val
ue
Adjusted p-val
ue
Odds
Ratio
Combined sco
re
1 Proton
Motive Force -Driven
Mitochondr
ial ATP Syn thesi
s (GO:0042776)
6.31E
-08
0.0001736
10.
42
172.
78
2 Proton
Motive Force -Driven
ATP Syn thesi
s (GO:0015986)
2.42E
-07
0.0002798
8.93
136.
03
3 Dica
rboxylic
Aci d Metabolic
Proce ss
(GO:0043648)
3.65E
-07
0.0002798
9.93
147.
17
4 Oxi dat
ive Phosphor
yl ation
(GO:0006119)
4.07E
-07
0.0002798
8.41
123.
8
5 Fatty
Aci d Metabolic
Proce ss
(GO:0006631)
0.000002252
0.001149
5.17
67.
26
6 Fatty
Aci d Catabolic
Proce ss
(GO:0009062)
0.000002506
0.001149
7.78
100.
37
7 Sodi
um Ion
Expor
t Acr oss
Plasm
a Membrane
(GO:0036376)
0.000006556
0.002068
28.
13
335.
72
8 Aerobi
c Elect ron
Transpor
t Cha
in (GO:0019646)
0.000006909
0.002068
6.84
81.
29
9 Potassi
um Ion I
mpor
t Acr oss
Plasm
a Membrane
(GO:1990573)
0.000008061
0.002068
9.31
109.
19
10 Cellu lar Resp iration
(GO:0045333)
0.000008591
0.002068
5.91
68.
88
11 Posi tive Regul
ation Of E
ndot
helia
l Cell Migration
(GO:0010595)
0.000008591
0.002068
5.91
68.
88
12 Mitochondr
ial ATP Syn thesi
s Coupl
ed
Elect ron
Transpor
t (GO:0042775)
0.000009016
0.002068
6.61
76.
81
13 Gluco se Metabolic
Proce ss
(GO:0006006)
0.00001469
0.003109
7.13
79.
35
14 Secondar
y Alcohol
Metabolic
Proce ss
(GO:1902652)
0.00001925
0.003784
8.11
88.
1
15 Proton
Transm
embrane
Transpor
t (GO:1902600)
0.00002642
0.004816
7.72
81.
35
16 ATP Biosyn
thet
ic
Proce ss
(GO:0006754)
0.000028
0.004816
12.
45
130.
55
17 Fatty
Aci d Oxi dat
ion
(GO:0019395)
0.00004129
0.006684
7.19
72.
58
18 Regul
ation Of H
ear
t Cont
ract ion
(GO:0008016)
0.00005311
0.008119
5.94
58.
46
19 Angi
ogenesi
s I nvo lved I
n Wound
Healing
(GO:0060055)
0.0000719
0.009894
26.
2
249.
97
20
Base ment
Membrane O
rgani
za tion
(GO:0071711)
0.0000719
0.009894
26.
2
249.
97
Biological Process GO
analysis for lamprey TH
+
neurons. Analysis is performed using the top 500 significantly upregulated transcripts with an enrichment cutoff of
1.5 log
2
(Fold Change). p-values are determined using Fisher’s exact test.