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Published January 2015 | public
Book Section - Chapter

Magnesium-embedded live cell filter for CTC isolation


This paper reports a novel Magnesium-embedded cell filter for Circulating Tumor Cell (CTC) capture, release and isolation. The new and novel feature is the use of thin-film Mg to release the captured CTCs based on the fact that any Cl^- containing culture medium can readily etch Mg away [1]. The releasing and the isolation of each individual CTC are demonstrated here. After filtration process, the filter is submerged in PBS to facilitate Mg etching. The top PA-C filter pieces break apart from the bottom after Mg completely dissolves, enabling captured CTC cells to detach from the filter. The released CTC can then be easily aspirated into a micropipette, and then for further, such as, DNA mutation analysis.

Additional Information

© 2015 IEEE. The authors gratefully acknowledge the help of all the members in California Institute of Technology (Caltech) Micromachining Lab, and University of Southern California (USC) Keck School of Medicine Norris Comprehensive Cancer Center, as well as experimental assistance of Mr. Trevor Roper.

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