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Published May 2006 | Published
Journal Article Open

Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy


Dual-wavelength reflection-mode photoacoustic microscopy is used to noninvasively obtain three-dimensional (3-D) images of subcutaneous melanomas and their surrounding vasculature in nude mice in vivo. The absorption coefficients of blood and melanin-pigmented melanomas vary greatly relative to each other at these two optical wavelengths (764 and 584nm). Using high-resolution and high-contrast photoacoustic imaging in vivo with a near-infrared (764-nm) light source, the 3-D melanin distribution inside the skin is imaged, and the maximum thickness of the melanoma (∼0.5mm) is measured. The vascular system surrounding the melanoma is also imaged with visible light (584nm) and the tumor-feeding vessels found. This technique can potentially be used for melanoma diagnosis, prognosis, and treatment planning.

Additional Information

© 2006 SPIE. Paper 06034LR received Feb. 22, 2006; revised manuscript received Apr. 17, 2006; accepted for publication Apr. 17, 2006; published online Jun. 14, 2006. This research was supported in part by the National Institutes of Health grants R01 EB000712 and R01 NS46214. J. T. Oh was supported in part by the postdoctoral Fellowship Program of Korea Science & Engineering Foundation (KOSEF).

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