In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog
Accurately locating epileptic foci has great importance in advancing the treatment of epilepsy. In this study, epileptic seizures were first induced by intracortical injection of 4-aminopyridine in rats. A fluorescent deoxyglucose substitute, 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG), was then continuously injected via the tail vein. Brain glucose metabolism was subsequently monitored by fluorescence imaging of 2-NBDG. The initial uptake rate of 2-NBDG at the injection site of 4-aminopyridine significantly exceeded that of the control injection site, which indicated local hypermetabolism induced by seizures. Our results show that 2-NBDG can be used for localizing epileptic foci.
Additional Information© 2011 Elsevier B.V. Received 26 April 2011; Received in revised form 6 September 2011; Accepted 7 September 2011. The authors thank Prof. James Ballard and Arie Krumholz for help in editing the manuscript, and Dr. Jianmin Cui and Mark Zaydman for their help with technical assistance. This work was supported by National Institutes of Health Grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, and 5P60 DK02057933 (L.V.W.) and GM077170 (A.V.D.). L.V.W has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Accepted Version - nihms325077.pdf