Three-dimensional photoacoustic imaging of subcutaneous microvasculature in vivo
Three-dimensional photoacoustic imaging of subcutaneous microvasculature in small animals is realized in vivo using photoacoustic microscopy. Previously, degraded lateral resolution in the out-of-focus region made three-dimensional visualization impractical. With the help of a virtual-detector synthetic aperture focusing technique, depth-independent lateral resolution is achieved. Therefore, volumetric imaging of the vessels is realized with a lateral resolution of 45 μm and an axial resolution of 15 μm. Detailed structural information, such as vessel depth and spatial orientation, are revealed with new clarity.
Additional Information© 2006 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank Ovidiu Craciun, Geng Ku, and Gina Lungu for experimental assistance. This project is sponsored by National Institutes of Health grants R01 EB000712 and R01 NS46214.
Published - 60861E.pdf