Transcriptional regulatory regions of testis-specific PGK2 defined in transgenic mice
The gene encoding testis-specific phosphoglycerate kinase 2 (PGK; ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 184.108.40.206) is expressed only in meiotic and haploid male germ cells. Transgenic mice containing an 8-kilobase human genomic PGK2 gene express the human gene in a tissue-specific and developmentally regulated manner. To determine the nature and location of sequences controlling this expression, transgenic mice with various lengths of the human PGK2 5' region fused to the chloramphenicol acetyltransferase (CAT) gene were analyzed for expression. A 323-base-pair region 5' to the coding region was found to contain information essential for both tissue-specific and developmentally regulated expression of the CAT reporter gene. Transgenic mice containing a PGK2/luciferase-coding constrct were compared with mice containing an equivalent CAT construct. Luciferase gene expression was also testis-specific and was more sensitive than CAT gene expression, but otherwise regulation of the two reporter genes was similar in the germ cells of transgenic mice. Translation of both PGK2/CAT and PGK2/luciferase fusion genes was seen concurrently with the first detectable transcripts.
Additional Information© 1989 by the National Academy of Sciences. Contributed by Melvin I. Simon, August 7, 1989. We thank Nathan Ellis for the gift of the mouse Pgk-2 3' clone and Jessie Dausman for assistance with the transgenic mice. This work was supported by a grant from the American Cancer Society to M.I.S. and from the Markey Foundation Grant for Developmental Biology to the California Institute of Technology as well as a Program Project Grant AG 07687 to M.I.S. Support was also provided by a National Institutes of Health Grant HD 23126 to J.R.M., who is a Career Development Fellow HD 00829. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Published - ROBpnas89.pdf