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Published May 15, 2000 | Version Published
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A Direct Interaction of Axonin-1 with NgCAM-related Cell Adhesion Molecule (NrCAM) Results in Guidance, but not Growth of Commissural Axons

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Abstract

An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti–axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.

Additional Information

© The Rockefeller University Press, 2000. Submitted: 20 December 1999. Revised: 22 March 2000. Accepted: 7 April 2000. We thank Ulrike Binkle and Claudia A.O. Stuermer for providing matrices for the stripe assay, Christine Mirzayan and Marc Tessier-Lavigne for providing the netrin-1–expressing cell line. We are indebted to Vance Lemmon for many stimulating discussions and helpful technical advice. We thank Ned Mantei for critical reading of the manuscript and Martin Zerner for help with statistics. This work was supported by grants of the Swiss National Science Foundation and the Biotechnology Programme of the European Union.

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Identifiers

PMCID
PMC2174557
Eprint ID
9786
Resolver ID
CaltechAUTHORS:FITjcb00

Funding

Swiss National Science Foundation (SNSF)
European Union

Dates

Created
2008-03-16
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Updated
2021-11-08
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