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Published 1994 | metadata_only
Book Section - Chapter

Metal affinity partitioning


The partitioning of biological materials in aqueous two-phase systems, for example, poly(ethylene glycol) (PEG)-dextran (Dx) systems, can be altered in a reproducible and predictable fashion using affinity ligands attached to one of the phase-forming polymers. Metal ion complexes such as copper(II) iminodiacetate [Cu(II)IDA] have several very attractive features as affinity ligands: they are stable under a wide range of solvent conditions and temperatures and are therefore easy to recycle; they can be incorporated into chromatographic supports with very high loading capacities; their interactions with target molecules are reasonably specific and are reversible under mild conditions; and they are inexpensive.

Additional Information

© 1994 by Academic Press, Inc. This research is supported by the National Science Foundation, Grant No. EET-8807351, and a Presidential Young Investigator Award (F.H.A.). F.H.A. gratefully acknowledges a fellowship from the David and Lucile Packard Foundation. D.B. is the recipient of a fellowship from the Irvine Foundation.

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August 20, 2023
August 20, 2023