The mitophagy receptors BNIP3 and NIX mediate tight attachment and expansion of the isolation membrane to mitochondria

We thank Tamotsu Yoshimori (Osaka University) for providing us with the HeLa Kyoto cells stably expressing mCherry-Parkin; the Division for Medical Research Engineering, Nagoya University Graduate School of Medicine, for providing us access to Amira software; Tatsuya Sugisaki, Wu Huajui, and Katsuyuki Kanno for their assistance with EM analyses; and all of the members of the Waguri laboratory for their valuable discussions. We also thank Ramaciotti Centre for Cryo-Electron Microscopy, with particular thanks to Gediminas Gervinskas for his technical assistance with FIB-scanning EM. Finally, we thank Michelle Kahmeyer-Gabbe, PhD, from Edanz (https://jp.edanz.com/ac) for editing a draft of this manuscript.

This work was supported by multiple JSPS KAKENHI grants (16KK0162 and 22K07207 to S.-i. Yamashita, 22K06300 to R. Arai, 24H02274 and 23K23878 to T. Kanki, and 23K27351 and 20H03415 to S. Waguri), an Japan Agency for Medical Research and Development Grant (JP24gm1710006 to T. Kanki), the Takeda Science Foundation (S.-i. Yamashita), the Suzuken Memorial Foundation (S.-i. Yamashita), and Rebecca Cooper Foundation Fellowship (RC20241396 to M. Lazarou). Open Access funding provided by Fukushima Medical University.

Author contributions: S.-i. Yamashita: conceptualization, formal analysis, funding acquisition, investigation, methodology, project administration, resources, validation, visualization, and writing—original draft, review, and editing. R. Arai: conceptualization, data curation, formal analysis, funding acquisition, investigation, methodology, project administration, supervision, validation, visualization, and writing—original draft, review, and editing. H. Hada: investigation and writing—review and editing. B.S. Padman: investigation. M. Lazarou: resources. D.C. Chan: resources, supervision, and writing—review and editing. T. Kanki: conceptualization, funding acquisition, methodology, project administration, resources, supervision, validation, and writing—review and editing. S. Waguri: conceptualization, data curation, formal analysis, funding acquisition, investigation, methodology, project administration, resources, software, supervision, validation, visualization, and writing—original draft, review, and editing.