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Published March 1978 | public
Journal Article

A technique of low-pH gel electrophoresis of chromosomal proteins which does not require preliminary removal of DNA


Fractionation of chromosomal proteins, in particular, of histones, by acetic acid-urea polyacrylamide gel electrophoresis usually requires preliminary removal of DNA from deoxyribonucleoprotein samples to obtain good separation of proteins. We have found that this difficulty can be overcome by addition of cetyltrimethylammonium bromide (CTAB) to the gel and electrode buffers. Since CTAB can readily diffuse into polyacrylamide gels two-dimensional fractionation becomes possible; that is, deoxyribonucleoprotein particles are fractionated in the first dimension followed by immersion of a gel in a CTAB solution and then low-pH gel electrophoresis of proteins in the second dimension.

Additional Information

© 1978 Academic Press. Received 1 June 1977, Accepted 8 September 1977. We thank Dr. V. A. Berdnikov for advice on the use of slab gels and Professor G. P. Georgiev and Dr. V. V. Bakayev for helpful discussions.

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