Supplementary
File
1
.
Example
calculations
of
synGAP/PSD
-
95
ratio
and
TARPs/PSD
-
95
ratio
for
animals
33
and
34.
The
Table
shows
the
steps
to
calculate
a
synGAP/PSD
-
95
ratio
and
a
TARPs/PSD
-
95
ratio
from
digital
data
recorded
from
one
pair
of
gels
containing
samples
from
animal
s
33
and
34
(see
Figure
1
-
figure
supplement
1).
Data
were
collected
from
at
least
3
technical
replicates
of
sy
nGAP
gel
lanes
for
each
animal
and
usually
6
technical
replicates
of
target
gel
lanes.
T
he
resulting
ratios
were
averaged.
Animal
ID
Signal
Intensity
Signal
Intensity
-
Bkg.
synGAP/PSD
-
95
Ratio
33
PSD95
6620000
2540000
0.489
33
PSD95_Background
4080000
33
SynGAP
1720000
1242000
33
SynGAP_Background
478000
34
PSD95
5800000
1970000
0.686
34
PSD95_Background
3830000
34
SynGAP
1800000
1351000
34
SynGAP_Background
449000
Animal
ID
Signal
Intensity
Signal
Intensity
-
Bkg.
TARPs/PSD
-
95
Ratio
33
PSD95
5460000
2560000
0.104
33
PSD95_Background
2900000
33
TARPs
1170000
265000
33
TARPs_Background
905000
34
PSD95
3880000
1760000
0.092
34
PSD95_Background
2120000
34
TARPs
853000
162000
34
TARPs_Background
691000