S1
Binding of Ru(bpy)
2
(eilatin)
2+
to Matched and Mismatched DNA
Brian M. Zeglis and Jacqueline K. Barton*
Division of Chemistry and Chemical Engineering,
California Institute of Technology, Pasadena, California, 91125
Supporting Information
S1.
Sample Rh
(bpy)
2
(chrysi)
3+
Binding Constant Determination Gel
S2.
Plot of Rh(bpy)
2
(chrysi)
3+
Cleavage Band Intensity as a Function of
Rh(bpy)
2
(chrysi)
3+
for Binding Constant Determination
S3.
Rh(bpy)
2
(chrysi)
3+
/Ru(bpy)
3
2+
Control Competition Experiment Gel
S2
1: Mismatched DNA Maxam Gilberts (AG)
2: Mismatched DNA Maxam Gilberts (CT)
3. 1
μ
M Mismatched DNA, light control, 5 min irradiation
4. 1 μ M Mismatched DNA, dark control
5: 1
μ
M Mismatched DNA, 0
μ
M Rh(bpy)
2
(chrysi), 5 min irradia
tion
6: 1
μ
M Mismatched DNA, 0.05
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
7: 1
μ
M Mismatched DNA, 0.1
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
8: 1
μ
M Mismatched DNA, 0.2
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
9: 1
μ
M Mismatched DNA, 0.3
μ
M Rh(bpy)
2
(chrysi),
5 min irradiation
10: 1
μ
M Mismatched DNA, 0.4
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
11: 1
μ
M Mismatched DNA, 0.5
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
12: 1
μ
M Mismatched DNA, 0.6
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
13: 1
μ
M Mismatched DNA, 0.7
μ
M Rh(
bpy)
2
(chrysi), 5 min irradiation
14: 1
μ
M Mismatched DNA, 0.8
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
15: 1
μ
M Mismatched DNA, 0.9
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
16: 1
μ
M Mismatched DNA, 1
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
17: 1
μ
M Mismatched D
NA, 2
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
18: 1
μ
M Mismatched DNA, 3
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
19: 1
μ
M Mismatched DNA, 4
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
20: 1
μ
M Mismatched DNA, 5
μ
M Rh(bpy)
2
(chrysi), 5 min irradiation
21. Mismatche
d DNA Maxam Gilberts (AG)
22. Mismatched DNA Maxam Gilberts (CT)
S1.
Sample Rh(bpy)
2
(chrysi)
3+
Binding Constant Determination Gel
S3
S2.
Plot of Rh(bpy)
2
(chrysi)
3+
Cleavage Band Intensity as a Function of
Rh(bpy)
2
(chrysi)
3+
for Binding Constan
t Determination
S4
1: Mismatched Maxam Gilberts (AG)
2. Mismatched Maxam Gilberts (CT)
3. 0.66 μ M Mismatched DNA, light control
4 0.66 μ M Mismatched DNA, 0.66 uM Rh(bpy)
2
(chrysi)
3+
,
0
μ
M Ru(bpy)
3
2+
, 6 minute irr.
5: 0.66 μ M Mismatc
hed DNA, 0.66 uM Rh(bpy)
2
(chrysi)
3+
,
0.1
μ
M Ru(bpy)
3
2+
, 6 minute irr.
6: 0.66 μ M Mismatched DNA, 0.66 uM Rh(bpy)
2
(chrysi)
3+
,
0.33
μ
M Ru(bpy)
3
2+
, 6 minute irr.
7: 0.66 μ M Mismatched DNA, 0.66 uM Rh(bpy)
2
(chrysi)
3+
,
1
μ
M Ru(bpy)
3
2+
, 6 minute irr.
8: 0.66
μ M Mismatched DNA, 0.66 uM Rh(bpy)
2
(chrysi)
3+
,
2
μ
M Ru(bpy)
3
2+
, 6 minute irr.
9: 0.66 μ M Mismatched DNA, 0.66 uM Rh(bpy)
2
(chrysi)
3+
,
3.3
μ
M Ru(bpy)
3
2+
, 6 minute irr.
10: 0.66 μ M Mismatched DNA, 0.66 uM Rh(bpy)
2
(chrysi)
3+
,
5
μ
M Ru(bpy)
3
2+
, 6 minute irr.
11: 0.66 μ M Mismatched DNA, 0.66 uM Rh(bpy)
2
(chrysi)
3+
,
15
μ
M Ru(bpy)
3
2+
, 6 minute irr.
12. 0.66 μ M Mismatched DNA, 0.66 uM Rh(bpy)
2
(chrysi)
3+
,
33
μ
M Ru(bpy)
3
2+
, 6 minute irr.
13: Mismatched Maxam Gilberts (AG)
14. Mismatched Maxam Gilberts (CT)
S3.
Rh(bp
y)
2
(chrysi)
3+
/Ru(bpy)
3
2+
Control Competition Experiment Gel