NMR evidence for specific intercalation of Δ-rh(phen)_2phi^(3+) in [d(GTCGAC)_2]
The anchoring of metal complexes in the major groove of DNA through intercalation has been increasingly useful in the shape-selective design of novel metal complexes which bind DNA with high sequence-selectivity. Toward that goal, direct structural information regarding this intercalative interaction is essential. Phenanthrenequinone diimine (phi) complexes of Rh(III) bind avidly (K_b 1 ≥ 10^7) to DNA by intercalation in the major groove. Here we report the first ^1H-NMR studies of Δ-rh(phen)_2phi^(3+) bound to an oligonucleotide. These studies provide direct structural evidence for specific intercalation by this octahedral complex in the major groove of DNA.
© 1993 American Chemical Society. Received December 15. 1992. We are grateful to the National Institutes of Health (GM33309 and an NRSA GM13861-03 to S.S.D.) for their financial support. In addition, we thank B. Imperiali for help in processing the NMR data.
Supplemental Material - ja00060a060_si_001.pdf
Supplemental Material - ja2984.pdf