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Published February 15, 2018 | Accepted Version
Journal Article Open

Label-free cell nuclear imaging by Grüneisen relaxation photoacoustic microscopy


Photoacoustic microscopy (PAM) with ultraviolet (UV) laser illumination has recently been demonstrated as a promising tool that provides fast, label-free, and multilayered histologic imaging of human breast tissue. Thus far, the axial resolution has been determined ultrasonically. To enable optically defined axial resolution, we exploit the Grüneisen relaxation (GR) effect. By imaging mouse brain slices, we show that GRUV-PAM reveals detailed information about three-dimensional cell nuclear distributions and internal structures, which are important diagnostic features for cancers. Due to the nonlinear effect, GRUV-PAM also provides better contrast in images of cell nuclei.

Additional Information

© 2018 Optical Society of America. Received 6 November 2017; revised 29 December 2017; accepted 14 January 2018; posted 22 January 2018 (Doc. ID 309604); published 15 February 2018. Funding: National Institutes of Health (NIH) (DP1 EB016986 NIH Director's Pioneer Award, R01 CA186567 NIH Director's Transformative Research Award). The authors appreciate Professor James Ballard's close reading of the Letter. Competing interests: L. V. W. has a financial interest in MicroPhotoAcoustics Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work.

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