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Published November 1984 | public
Journal Article

A trans-acting regulatory product necessary for expression of the Drosophila melanogaster 68C glue gene cluster


The mutation I(1)npr-1 is located at cytological location 2B5 on the X chromosome in Drosophila melanogaster. We have found that this mutation causes absence of the normal product of the 2B5 locus and that it has the following phenotypes: the 68C glue puff on the third chromosome does not regress when mutant salivary glands are cultured in the presence of ecdysterone; the three 68C glue protein mRNAs are not synthesized; and a transformed Drosophila strain carrying both a normal resident 68C Sgs-3 gene and an introduced functional Sgs-3 gene with only a few kb of flanking sequences expresses neither Sgs-3 RNA if the I(1)npr-1 mutation is crossed into the stock. Thus the normal product of the I(1)npr11 gene is required for regression of the 68C puff, and the I(1)npr-1 gene product allows expression of the Sgs-3 gene by interacting, either directly or indirectly, with DNA sequences near this glue protein gene.

Additional Information

© 1984 MIT. Received March 19, 1984, revised July 31, 1984. We are grateful to Drs. James Fristrom, lstvan Kiss, and Igor Zhimulev for supplying the mutant Drosophila strains with which the experiments were performed, and to Dr. Greg Guild for providing the Group V gene clone. We wish to thank the other members of our lab for critical comments on, and discussions of, the manuscript. This work was supported by grant GM 28075 awarded to E. M. M. by the Institute of General Medical Sciences, National Institutes of Health. T. E. C and P. H. M. were supported by National Research Service Awards (1 T32 GM07616) from the National Institutes of Health and by Helen G. and Arthur McCallum Fellowships. During part of this work T. E. C. was also supported by a California Foundation for Biochemical Research Fellowship. The costs of publication of this article were defrayed in part by the payment of page charges. Thus article must therefore be hereby marked "advertisement" In accordance with 18 U.S.C. Section 1734 solely to indicate thus fact.

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