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Published August 15, 1982 | Published
Journal Article Open

Regulated expression of an extrachromosomal human β-interferon gene in mouse cells


β (fibroblast)-interferon mRNA and protein are induced by the synthetic double-stranded RNA poly(I)·poly(C) in cultured human fibroblasts. To study the mechanism of this induction, we have isolated a human β-interferon gene and inserted it in a vector plasmid containing DNA of the bovine papilloma virus. After removal of bacterial plasmid sequences, the bovine papilloma virus β-interferon recombinant was used to morphologically transform mouse fibroblasts. Analysis of DNA from the transformed cell lines indicated that this recombinant is propagated as a stable multicopy extrachromosomal element. Human β-interferon mRNA and protein are inducible by poly(I)·poly(C) in all of these cell lines, and the mRNA is indistinguishable from β-interferon mRNA synthesized by induced human cells.

Additional Information

Copyright © 1982 by the National Academy of Sciences. Contributed by Mark Ptashne, May 10, 1982. We thank Daniel DiMaio, Brian Seed, Tadatsugu Taniguchi, John Douhan III, Roger Brent, and Egon Amann for helpful discussions and materials and Elizabeth Leahy for expert technical assistance. K.Z. was supported by a National Science Foundation Predoctoral Fellowship. P.M. was supported by a National Institutes of Health Postdoctoral Fellowship. This work was supported by grants from the National Science Foundation and the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

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