Supporting Information
for
Adv. Sci.
, DOI 10.1002/advs.202202907
Long-Duration and Non-Invasive Photoacoustic Imaging of Multiple Anatomic
al Structures
in a Live Mouse Using a Single Contrast Agent
Anjul Khadria, Chad D. Paavola, Yang Zhang, Samuel P. X. Davis,
Patrick F. Grealish,
Konstantin Maslov, Junhui Shi, John M. Beals*, Sunday S. Oladipupo* a
nd Lihong V. Wang*
Supplementary information
Long
-
duration and non
-
invasive photoacoustic imaging of multiple anatomical
structures in a live mouse using a single contrast agent
Anjul Khadria
1
, Chad D. Paavola
2
, Yang Zhang
1
,
Samuel P. X. Davis
1
,
Patrick Grealish
2
,
Konstantin Maslov
1
, Junhui Shi
1
, John M. Beals
3
*, Sunday S
.
Oladipupo
2
*, Lihong V.
Wang
1
*
1
Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical
Engineering, Department of Electrical Engineering, California Institute of Techno
logy,
Pasadena,
California, 91125, USA
2
Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis,
Indiana, 46285, USA
3
Lilly Research Laboratories, Eli Lilly and Company, Lilly Biotechnology Center, San Diego,
California, 9
2121, USA
Figure
S1
:
O
verlay of
sulfo
-
cy
7.5 labeled (light blue) and unlabeled (dark blue)
IgG4
isotype control
antibody.
Figure
S
2
:
Long
-
duration
imaging
of multiple biological features in mouse ear skin after injection of
dye
-
labeled monoclonal IgG4 isotype control antibody. All color bars
represent normalized
photoacoustic amplitude and
range from 0 to 1. Scale bar, 500 μm.
Figure
S
3
:
Detection
of signals from dye
-
labeled IgG4 antibody ev
e
n after 50 days of injection
confirmin
g
its
slow clearance rate.
Scale bar, 500 μm.
Figure
S
4
:
Visualization of deep medial lymphatic vessel and lymph node
in the mouse leg and thigh
18
5
minutes after the injection of dye
-
labeled IgG4 antibody in mouse hind
-
paw
using PACT
.
LV,
lymphatic vessel; LN, lymphatic node
; PA, photoacoustic
.
Scale bar
,
0.4 cm.