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Published April 6, 2014 | Supplemental Material
Journal Article Open

Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting


We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section. Furthermore, the scattered-light-induced background is significantly reduced, considerably improving image contrast. Our method is expected to expand the application field of LSFM into the optical quasi-ballistic regime, enabling studies on non-transparent biological samples.

Additional Information

© 2014 The Author(s). Published by the Royal Society. Received: 19 September 2013; Accepted: 7 January 2014; Published 29 January 2014. We thank James Ballard for careful reading of the manuscript. We thank David Lyons (University of Edinburgh) for the transgenic zebrafish. We also thank Sarah DeGenova and Kelly Monk for helping prepare the zebrafish embryo sample. This work was sponsored in part by National Institutes of Health (NIH) grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA134539 and R01 CA159959. L. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

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