Supplementary Material
DNA Oxidation by Charge Transport in Mitochondria#
Edward J. Merino and Jacqueline K. Barton*
Division of Chemistry and Chemical Engineering,
California Institute of Technology, Pasadena, CA 91125
jkbarton@caltech.edu
Figure S1
. PAGE gel showing primer extension of functioning mitochondria upon
treatment with proteinase K. Functioning mitochondria in homogenized cell were
purified as described in the text. The homogenized cells (200
μ
L) were treated with 2
μ
g
of proteinase K at 37
2
C for 30 minutes. Primer extension of untreated homogenized cells
and subsequent primer extension yields the same repetitive stop pattern. Addition of
proteinase K gives a new uninterrupted primer extension product (red triangle) that
corresponds to DNA without bound protein. This indicates that the stop pattern is
dependent on protein within the mitochondria.
Supplementary Figure S1